Document Detail


Endothelial cell responses to micropillar substrates of varying dimensions and stiffness.
MedLine Citation:
PMID:  22389314     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the vascular niche, the extracellular matrix (ECM) provides a structural scaffold with a rich ligand landscape of essential matrix proteins that supports the organization and stabilization of endothelial cells (ECs) into functional blood vessels. Many of the physical interactions between ECs and macromolecular components of the ECM occur at both the micron and submicron scale. In addition, the elasticity of the ECM has been shown to be a critical factor in the progress of the angiogenic cascade. Here, we sought to determine the effect of substrate topography and elasticity (stiffness) on EC behavior. Utilizing a unique SiO(2) substrate with an array of micropillars, we first demonstrate that micropillars with heights >3 μm significantly decrease EC adhesion and spreading. Fibronectin (Fn) patterning of 1 μm high micropillars enabled EC adhesion onto the micropillars and promoted alignment in a single-cell chain manner. We then developed a robust method to generate a soft micropillar substrate array made of polydimethylsiloxane (PDMS), similar to the SiO(2) substrate. Finally, we examined the kinetics of EC adhesion and spreading on the soft PDMS substrates compared to the stiff SiO(2) substrates. Culturing cells on the PDMS substrates demonstrated an enhanced EC elongation and alignment when compared to stiff SiO(2) with similar topographical features. We conclude that the elongation and alignment of ECs is coregulated by substrate topography and stiffness and can be harnessed to guide vascular organization.
Authors:
Laura E Dickinson; Danielle R Rand; Joanna Tsao; Wolfgang Eberle; Sharon Gerecht
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-03-03
Journal Detail:
Title:  Journal of biomedical materials research. Part A     Volume:  100     ISSN:  1552-4965     ISO Abbreviation:  J Biomed Mater Res A     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-04-11     Completed Date:  2012-08-01     Revised Date:  2014-09-24    
Medline Journal Info:
Nlm Unique ID:  101234237     Medline TA:  J Biomed Mater Res A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1457-66     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
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MeSH Terms
Descriptor/Qualifier:
Cell Adhesion
Cell Line
Coated Materials, Biocompatible / chemistry*
Dimethylpolysiloxanes / chemistry
Elasticity
Endothelial Cells / cytology*
Fibronectins / chemistry
Humans
Silicon Dioxide / chemistry*
Surface Properties
Tissue Scaffolds / chemistry*
Grant Support
ID/Acronym/Agency:
U54 CA143868/CA/NCI NIH HHS; U54 CA143868-03/CA/NCI NIH HHS; U54CA143868/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Coated Materials, Biocompatible; 0/Dimethylpolysiloxanes; 0/Fibronectins; 7631-86-9/Silicon Dioxide
Comments/Corrections

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