| Endothelial cell-derived nitric oxide mobilization is attenuated in copper-deficient rats. | |
| | |
MedLine Citation:
|
PMID: 19088764 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The attenuation of endothelium-dependent nitric oxide (NO) mediated vasodilation is a consistent finding in both conduit and resistance vessels during dietary copper (Cu) deficiency. Although the effect is well established, evidence for the mechanism remains circumstantial. This study was designed to determine the relative amount of NO produced in and released from the vascular endothelium. Using the fluorescent NO indicator, 4-amino-5-methylamino-2',7'-difluorofluorescein (DAF-FM), we now demonstrate the effect of a Cu-deficient diet on the production of NO from the endothelium of resistance arterioles. In one group of experiments, control and Cu-chelated lung microvascular endothelial cells (ECs) were used to assay NO production and fluorescence was observed by confocal microscopy. Weanling Sprague-Dawley rats were fed purified diets that were either Cu adequate (6.3 micrograms Cu per gram of food) or Cu deficient (0.3 micrograms Cu per gram of food) for 4 weeks. In the second series of experiments, first-order arterioles were microsurgically isolated from the rat cremaster muscle, cannulated, and pressurized with (3[N-morpholino]propanesulfonic acid) physiologic salt solution (MOPS-PSS). DAF-FM (5 micromol.L-1) was added in the lumen of the vessel to measure NO release. Baseline DAF-FM fluorescence was significantly lower in Cu-chelated ECs than in controls. In response to 10-6 mol.L-1 acetylcholine, fluorescent intensity was significantly less in chelated ECs and in the lumen of Cu-deficient arterioles. The results suggest that production and release of NO by the vascular endothelium is inhibited by a restriction of Cu. This inhibition may account for the attenuated vasodilation previously reported in Cu-deficient rats. |
| | |
Authors:
|
Jeff C Falcone; David Lominadze; W Thomas Johnson; Dale A Schuschke |
Related Documents
:
|
16361504 - The effects of high levels of supplemental copper on the serum lipid profile, carcass t... 2484424 - Effect of dietary copper on vanadate toxicity in chicks. 9521454 - The effect of dietary supplementation with copper sulfate or tribasic copper chloride o... 8169654 - Pool size and concentration of plasma cholesterol are increased and tissue copper level... 20122814 - Increased maillard reaction products intake reduces phosphorus digestibility in male ad... 20962914 - Effects of a highly palatable diet on lipid and glucose parameters, nitric oxide, and e... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
|
Title: Applied physiology, nutrition, and metabolism = Physiologie appliquée, nutrition et métabolisme Volume: 33 ISSN: 1715-5312 ISO Abbreviation: Appl Physiol Nutr Metab Publication Date: 2008 Dec |
Date Detail:
|
Created Date: 2008-12-17 Completed Date: 2009-03-24 Revised Date: 2011-01-14 |
Medline Journal Info:
|
Nlm Unique ID: 101264333 Medline TA: Appl Physiol Nutr Metab Country: Canada |
Other Details:
|
Languages: eng Pagination: 1073-8 Citation Subset: IM |
Affiliation:
|
Department of Physiology and Biophysics, University of Louisville School of Medicine, Louisville, KY 40292, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Analysis of Variance Animals Arterioles / drug effects, metabolism Copper / administration & dosage, deficiency* Diet / methods Endothelial Cells / metabolism* Endothelium, Vascular / metabolism* Male Microcirculation / drug effects Muscle, Skeletal / blood supply Nitric Oxide / biosynthesis*, metabolism Rats Rats, Sprague-Dawley Time Factors Vasodilation / drug effects |
| Grant Support | |
ID/Acronym/Agency:
|
DK55030/DK/NIDDK NIH HHS; R01 DK055030-08/DK/NIDDK NIH HHS; R01 HL080394-03/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
10102-43-9/Nitric Oxide; 7440-50-8/Copper |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Plasma deuterium oxide accumulation following ingestion of different carbohydrate beverages.
Next Document: Caffeine and carbohydrate supplementation during exercise when in negative energy balance: effects o...