Document Detail


Endothelial nitric oxide synthase genotypes in the etiology of retinopathy of prematurity in premature infants.
MedLine Citation:
PMID:  20809776     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: Retinopathy of Prematurity (ROP) is a vasoproliferative disorder affecting preterm infants leading to visual impairment. ROP is more common in Caucasians than African Americans. Very low birth weight infants have immature retinas and are susceptible to ROP. Because of differences in individual responses to the treatment, various genetic factors have been looked into to understand the etiology of ROP. Endothelial nitric oxide (eNO) serves as a vasodilator, relaxes smooth muscle, prevents platelet aggregation, and facilitates improved blood flow and vascular tonicity. Mutant eNO synthase (eNOS) genotypes result in reduced nitric oxide levels by decreasing enzyme activity. Since eNO affects vasculature and ROP is a vascular disease, the present investigation was aimed at studying the association of genotypes with ROP.
METHODS: Two eNOS gene single nucleotide polymorphisms (SNPs) (T-786C, and G894T) were studied by microplate-Restriction Fragment Length Polymorphism Polymerase Chain Reaction (RFLP PCR) method. Genotypes were studied in 146 premature infants.
RESULTS: The present data showed significant differences in the baseline gene frequencies between Caucasians and African Americans. ROP patients displayed 3-fold higher frequencies of mutant -786C and 894T alleles in both ethnicities compared to respective controls.
CONCLUSIONS: The present data suggest ethnic stratification of genotypes. Mutant -786C and 894T alleles are significant risk factors in the development of ROP, and suggest a strong association between eNOS polymorphisms and the disease. It is interesting to know if a larger dataset of ROP patients can confirm our initial findings.
Authors:
Krishna Yanamandra; Dawn Napper; Arun Pramanik; Joseph A Bocchini; Ramasubbareddy Dhanireddy
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-09-01
Journal Detail:
Title:  Ophthalmic genetics     Volume:  31     ISSN:  1744-5094     ISO Abbreviation:  Ophthalmic Genet.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-11-11     Completed Date:  2011-01-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9436057     Medline TA:  Ophthalmic Genet     Country:  England    
Other Details:
Languages:  eng     Pagination:  173-7     Citation Subset:  IM    
Affiliation:
Department of Pediatrics, Louisiana State University Health Sciences Center, Shreveport, Louisiana 71103, USA. kyanam@lsuhsc.edu
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MeSH Terms
Descriptor/Qualifier:
African Americans
European Continental Ancestry Group
Gene Frequency
Genotype
Gestational Age
Humans
Infant, Newborn
Infant, Premature*
Nitric Oxide / metabolism
Nitric Oxide Synthase Type III / genetics*
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Polymorphism, Single Nucleotide*
Retinopathy of Prematurity / enzymology,  genetics*
Chemical
Reg. No./Substance:
10102-43-9/Nitric Oxide; EC 1.14.13.39/NOS3 protein, human; EC 1.14.13.39/Nitric Oxide Synthase Type III

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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