Document Detail

Endothelial Cells Mediate Islet-specific Maturation of Human Embryonic Stem Cell Derived Pancreatic Progenitor Cells.
MedLine Citation:
PMID:  24943736     Owner:  NLM     Status:  Publisher    
It is well recognized that in-vitro differentiation of embryonic stem cells (ESC) can be best achieved by closely recapitulating the in-vivo developmental niche. Thus implementation of directed differentiation strategies has yielded encouraging results in the area of pancreatic islet differentiation. These strategies have concentrated on direct addition of chemical signals, however, other aspect of the developmental niche are yet to be explored. During development, pancreatic progenitor (PP) cells grow as an epithelial sheet, which aggregates with endothelial cells (EC) during the final stages of maturation. Several findings suggest that the interactions with EC play a role in pancreatic development. In this study we recapitulated this phenomenon in an in-vitro environment by maturing the hESC-derived PP cells in close contact with EC. We find that co-culture with different EC (but not fibroblast) alone results in pancreatic islet-specific differentiation of hESC-derived PP cells even in the absence of additional chemical induction. The differentiated cells responded to exogenous glucose levels by enhanced C-peptide synthesis. The co-culture system aligned well with endocrine development as determined by comprehensive analysis of involved signaling pathways. By recapitulating cell-cell interaction aspects of the developmental niche we achieved a differentiation model that aligns closely with islet organogenesis.
Maria Jaramillo; Shibin Mathew; Hikaru Mamiya; Saik Kia Goh; Ipsita Banerjee
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-6-18
Journal Detail:
Title:  Tissue engineering. Part A     Volume:  -     ISSN:  1937-335X     ISO Abbreviation:  Tissue Eng Part A     Publication Date:  2014 Jun 
Date Detail:
Created Date:  2014-6-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101466659     Medline TA:  Tissue Eng Part A     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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