Document Detail


Endothelial Barrier Dysfunction in Diabetic Conduit Arteries: A Novel Method to Quantify Filtration.
MedLine Citation:
PMID:  23220330     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The endothelial barrier plays an important role in atherosclerosis, hyperglycemia, and hypercholesterolemia. An accurate, reproducible, and user-friendly method is helpful to further understand endothelial barrier function of conduit arteries. An isovolumic method is used to measure the hydraulic conductivity of intact vessel wall and medial-adventitial layer. Normal arterial segments with diameters from 0.2 mm to 5.5 mm were used to validate the method and femoral arteries of diabetic rats were studied as an example of pathological specimens. Various arterial segments confirmed that the volume flux (J(v)) of water per unit surface area (S) is linearly related to intraluminal pressure as is well-established in microvessels. The hydraulic conductivity (L(p)) of intact wall varied from 3.5 to 22.1 ×10(-7) cm⋅s(-1)⋅cmH(2)O(-1) over the pressure range of 7 to 180 mmHg. Over the same pressure range, the L(p) of endothelial barrier changed from 4.4 to 25.1 ×10(-7) cm⋅s(-1)⋅cmH(2)O(-1). During perfusion with albumin-free solution, Lp of femoral artery of rat increased from 6.1 to 13.2 ×10(-7) cm⋅s(-1)⋅cmH(2)O(-1) over the pressure range of 10 to 180 mmHg. Hyperglycemia increased hydraulic conductivity of femoral artery in diabetic rats from 2.9 to 5.5 ×10(-7) cm⋅s-1⋅cmH(2)O(-1) over the pressure range of 20 to 135 mmHg. The hydraulic conductivity of conduit artery can be accurately and reproducibly measured using a novel isovolumic method. The conduit artery in diabetic rat is hyper-permeable likely due to disruption of endothelial glycocalyx as is known in the microvasculature.
Authors:
Xiao Lu; Virginia H Huxley; Ghassan S Kassab
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-7
Journal Detail:
Title:  American journal of physiology. Heart and circulatory physiology     Volume:  -     ISSN:  1522-1539     ISO Abbreviation:  Am. J. Physiol. Heart Circ. Physiol.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100901228     Medline TA:  Am J Physiol Heart Circ Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
1Indiana University-Purdue University Indianapolis.
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