| Endostar induces apoptotic effects in HUVECs through activation of caspase-3 and decrease of Bcl-2. | |
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MedLine Citation:
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PMID: 19331180 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Endostar is a novel recombinant human endostatin expressed and purified in Escherichia coli with the N-terminal modified. It has been shown that endostar inhibited the proliferation of human umbilical vein endothelial cells (HUVECs) through the membrane surface receptor KDR/flt-1(VEGFR-2, vascular endothelial growth factor receptor-2) by exerting anti-angiogenesis effects. But the molecular mechanism remained unclear. MATERIALS AND METHODS: The apoptotic effects induced by endostar in the serum-deprived situation were investigated by 4'-6-diaminidino-2-phenylindole (DAPI) staining and the Annexin V-fluorescein isothiocyanate (FITC) binding assay. The mechanism of action was explored by Western blotting assay. RESULTS: Endostar induced remarkable apoptosis in HUVECs. The expressions of apoptosis-related proteins showed that caspase-3 was activated, but caspase-8, a marker of the non-mitochondria-mediated apoptosis signal pathway, was not. Further investigation revealed that cellular Bcl-2 decreased in the endostar-treated groups, while the level of Bax was almost unchanged. CONCLUSION: Endostar induces apoptotic effects in HUVECs through the activation of caspase-3 and a decrease of the Bcl-2 to Bax ratio. |
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Authors:
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Yun Ling; Na Lu; Ying Gao; Yan Chen; Sen Wang; Yong Yang; Qinglong Guo |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Anticancer research Volume: 29 ISSN: 0250-7005 ISO Abbreviation: Anticancer Res. Publication Date: 2009 Jan |
Date Detail:
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Created Date: 2009-03-31 Completed Date: 2009-04-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8102988 Medline TA: Anticancer Res Country: Greece |
Other Details:
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Languages: eng Pagination: 411-7 Citation Subset: IM |
Affiliation:
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Jiangsu Key Laboratory of Carcinogenesis and Intervention, China Pharmaceutical University, Nanjing 210009 People's Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Caspase 3
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metabolism* Cells, Cultured Endostatins / pharmacology* Endothelial Cells / cytology, drug effects*, metabolism Enzyme Activation / drug effects Humans Proto-Oncogene Proteins c-bcl-2 / biosynthesis* Recombinant Proteins / pharmacology bcl-2-Associated X Protein / biosynthesis |
| Chemical | |
Reg. No./Substance:
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0/BAX protein, human; 0/Endostatins; 0/Proto-Oncogene Proteins c-bcl-2; 0/Recombinant Proteins; 0/bcl-2-Associated X Protein; 0/endostar protein; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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