Document Detail

Endosomal clathrin drives actin accumulation at the immunological synapse.
MedLine Citation:
PMID:  21321329     Owner:  NLM     Status:  In-Data-Review    
Antigen-specific cognate interaction of T lymphocytes with antigen-presenting cells (APCs) drives major morphological and functional changes in T cells, including actin rearrangements at the immune synapse (IS) formed at the cell-cell contact area. Here we show, using cell lines as well as primary cells, that clathrin, a protein involved in endocytic processes, drives actin accumulation at the IS. Clathrin is recruited towards the IS with parallel kinetics to that of actin. Knockdown of clathrin prevents accumulation of actin and proteins involved in actin polymerization, such as dynamin-2, the Arp2/3 complex and CD2AP at the IS. The clathrin pool involved in actin accumulation at the IS is linked to multivesicular bodies that polarize to the cell-cell contact zone, but not to plasma membrane or Golgi complex. These data underscore the role of clathrin as a platform for the recruitment of proteins that promote actin polymerization at the interface of T cells and APCs.
Carmen Calabia-Linares; Javier Robles-Valero; Hortensia de la Fuente; Manuel Perez-Martinez; Noa Martín-Cofreces; Manuel Alfonso-Pérez; Cristina Gutierrez-Vázquez; María Mittelbrunn; Sales Ibiza; Francisco R Urbano-Olmos; Covadonga Aguado-Ballano; Carlos Oscar Sánchez-Sorzano; Francisco Sanchez-Madrid; Esteban Veiga
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of cell science     Volume:  124     ISSN:  1477-9137     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-02-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  820-30     Citation Subset:  IM    
Servicio de Inmunología, Hospital Universitario de la Princesa, Instituto de Investigación Sanitaria Hospital de la Princesa (IP), Diego de León, 62, 28006 Madrid, Spain.
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