Document Detail

Endoscopic Raman spectroscopy enables objective diagnosis of dysplasia in Barrett's esophagus.
MedLine Citation:
PMID:  23886354     Owner:  NLM     Status:  Publisher    
BACKGROUND: Early detection and targeted endoscopic resection of Barrett's esophagus-associated high-grade dysplasia (HGD) can prevent progression to invasive esophageal malignancy. Raman spectroscopy, a highly sophisticated analytical technique, has been translated into an endoscopic tool to facilitate rapid, objective diagnosis of dysplasia in the esophagus.
OBJECTIVE: To evaluate the ability of endoscopic Raman spectroscopy (ERS) to objectively detect esophageal HGD and adenocarcinoma.
DESIGN: A total of 798 one-second spectra were measured from 673 ex vivo esophageal tissue samples, collected from patients with Barrett's esophagus by using a novel endoscopic Raman probe. Spectra were correlated with consensus histopathology. Multivariate analysis was used to evaluate the classification accuracy of ERS ex vivo.
SETTING: Probe measurements were conducted in the laboratory. Tissue specimens were collected from the operating theatre and endoscopy unit.
PATIENTS: Tissue from 62 patients was included in the study.
INTERVENTIONS: Endoscopic biopsy/resection or esophagectomy was performed where indicated clinically.
MAIN OUTCOME MEASUREMENT: Diagnostic performance of ERS for detection of HGD and esophageal adenocarcinoma.
RESULTS: ERS demonstrated a sensitivity of 86% and a specificity of 88% for detecting HGD and adenocarcinoma. The ability to grade dysplasia and differentiate intestinal metaplasia from nonintestinal metaplasia columnar-lined esophagus was also demonstrated. Diagnostic classification was based on objective measurement of the biochemical profile of different tissue types. The potential for combination ERS and narrow-band imaging was also demonstrated.
LIMITATIONS: Measurements were taken from ex vivo tissue.
CONCLUSION: ERS enables rapid, accurate, objective diagnosis of superficial esophageal disease (metaplasia, dysplasia, intramucosal cancer) in clinically applicable time scales.
L Max Almond; Jo Hutchings; Gavin Lloyd; Hugh Barr; Neil Shepherd; John Day; Oliver Stevens; Scott Sanders; Martin Wadley; Nick Stone; Catherine Kendall
Related Documents :
9339134 - Ebv-elicited familial hemophagocytic lymphohistiocytosis.
20496274 - Haemorrhoids or anal melanoma, importance of preoperative histopathological examination...
455584 - Vindesine as a single agent in the treatment of advanced malignant melanoma.
9181344 - Normal electro-oculograms in two patients with malignant melanoma of the choroid.
655924 - Epibulbar mucogenic subconjunctival cysts.
21956544 - Marsupialization for lacrimal ductular cysts (dacryops): a case series.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-7-22
Journal Detail:
Title:  Gastrointestinal endoscopy     Volume:  -     ISSN:  1097-6779     ISO Abbreviation:  Gastrointest. Endosc.     Publication Date:  2013 Jul 
Date Detail:
Created Date:  2013-7-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0010505     Medline TA:  Gastrointest Endosc     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013 American Society for Gastrointestinal Endoscopy. Published by Mosby, Inc. All rights reserved.
Biophotonics Research Unit, Gloucestershire Hospitals NHS Foundation Trust, Gloucester, United Kingdom; Department of Esophagogastric Surgery, Gloucestershire Hospitals NHS Foundation Trust, Gloucester, United Kingdom.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  EUS-guided biliary drainage by using a standardized approach for malignant biliary obstruction: rend...
Next Document:  Mapping histologic patchiness of celiac disease by push enteroscopy.