Document Detail


Endoplasmic reticulum targeted GFP reveals ER organization in tobacco NT-1 cells during cell division.
MedLine Citation:
PMID:  16647266     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The endoplasmic reticulum (ER) of plant cells undergoes a drastic reorganization during cell division. In tobacco NT-1 cells that stably express a GFP construct targeted to the ER, we have mapped the reorganization of ER that occurs during mitosis and cytokinesis with confocal laser scanning microscopy. During division, the ER and nuclear envelope do not vesiculate. Instead, tubules of ER accumulate around the chromosomes after the nuclear envelope breaks down, with these tubules aligning parallel to the microtubules of the mitotic spindle. In cytokinesis, the phragmoplast is particularly rich in ER, and the transnuclear channels and invaginations present in many interphase cells appear to develop from ER tubules trapped in the developing phragmoplast. Drug studies, using oryzalin and latrunculin to disrupt the microtubules and actin microfilaments, respectively, demonstrate that during division, the arrangement of ER is controlled by microtubules and not by actin, which is the reverse of the situation in interphase cells.
Authors:
S L Gupton; D A Collings; N S Allen
Related Documents :
11807936 - Over-expression of betai tubulin in mdck cells and incorporation of exogenous betai tub...
1915296 - A 210 kda nuclear matrix protein is a functional part of the mitotic spindle; a microin...
2879726 - Microtubules and the gonadotropic regulation of granulosa cell steroidogenesis.
3396056 - An ultrastructural study of mitosis and cytokinesis in normal 'resting' human breast.
19433456 - Regulators of b-cell activity in sle: a better target for treatment than b-cell depletion?
7996546 - Bioreductive markers for hypoxic cells: 2-nitroimidazoles with biotinylated 1-substitue...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-04-04
Journal Detail:
Title:  Plant physiology and biochemistry : PPB / Société française de physiologie végétale     Volume:  44     ISSN:  0981-9428     ISO Abbreviation:  Plant Physiol. Biochem.     Publication Date:    2006 Feb-Mar
Date Detail:
Created Date:  2006-06-07     Completed Date:  2007-04-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9882449     Medline TA:  Plant Physiol Biochem     Country:  France    
Other Details:
Languages:  eng     Pagination:  95-105     Citation Subset:  IM    
Affiliation:
Department of Botany, North Carolina State University, Raleigh, NC 27695, USA. stephg@scripps.edu
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Actins / metabolism
Cell Line
Chromosomes, Plant / physiology
Cytokinesis / physiology*
Endoplasmic Reticulum / metabolism*
Green Fluorescent Proteins / metabolism*
Interphase / physiology
Nuclear Envelope / metabolism
Tobacco / cytology*,  metabolism
Chemical
Reg. No./Substance:
0/Actins; 147336-22-9/Green Fluorescent Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Locus prl5 improves lodging resistance of rice by delaying senescence and increasing carbohydrate re...
Next Document:  Pharmacological evidence for activation of phospholipid and small GTP binding protein signalling cas...