Document Detail

Endoplasmic reticulum targeted GFP reveals ER organization in tobacco NT-1 cells during cell division.
MedLine Citation:
PMID:  16647266     Owner:  NLM     Status:  MEDLINE    
The endoplasmic reticulum (ER) of plant cells undergoes a drastic reorganization during cell division. In tobacco NT-1 cells that stably express a GFP construct targeted to the ER, we have mapped the reorganization of ER that occurs during mitosis and cytokinesis with confocal laser scanning microscopy. During division, the ER and nuclear envelope do not vesiculate. Instead, tubules of ER accumulate around the chromosomes after the nuclear envelope breaks down, with these tubules aligning parallel to the microtubules of the mitotic spindle. In cytokinesis, the phragmoplast is particularly rich in ER, and the transnuclear channels and invaginations present in many interphase cells appear to develop from ER tubules trapped in the developing phragmoplast. Drug studies, using oryzalin and latrunculin to disrupt the microtubules and actin microfilaments, respectively, demonstrate that during division, the arrangement of ER is controlled by microtubules and not by actin, which is the reverse of the situation in interphase cells.
S L Gupton; D A Collings; N S Allen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-04-04
Journal Detail:
Title:  Plant physiology and biochemistry : PPB / Société française de physiologie végétale     Volume:  44     ISSN:  0981-9428     ISO Abbreviation:  Plant Physiol. Biochem.     Publication Date:    2006 Feb-Mar
Date Detail:
Created Date:  2006-06-07     Completed Date:  2007-04-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9882449     Medline TA:  Plant Physiol Biochem     Country:  France    
Other Details:
Languages:  eng     Pagination:  95-105     Citation Subset:  IM    
Department of Botany, North Carolina State University, Raleigh, NC 27695, USA.
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MeSH Terms
Actins / metabolism
Cell Line
Chromosomes, Plant / physiology
Cytokinesis / physiology*
Endoplasmic Reticulum / metabolism*
Green Fluorescent Proteins / metabolism*
Interphase / physiology
Nuclear Envelope / metabolism
Tobacco / cytology*,  metabolism
Reg. No./Substance:
0/Actins; 147336-22-9/Green Fluorescent Proteins

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