Document Detail


Endoplasmic reticulum resident, immunoglobulin joining chain, can be secreted by perturbation of the calcium concentration in the endoplasmic reticulum.
MedLine Citation:
PMID:  15294089     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We established a transient human joining (J)-chain gene expression system in the baby hamster kidney (BHK) cell. The J-chain was detected as a 29-kDa single band on Western blotting. Immunofluorescent staining of the transfectant revealed an exclusive localization of the J-chain in the endoplasmic reticulum (ER). Intracellular transport experiment revealed that incubating conditions favorable for vesicular stomatitis virus glycoprotein (VSV-G) transport did not allow the J-chain to exit from the ER. Analysis of glycosylation status of the J-chain in the transfectant was examined by tunicamycin treatment, endoglycosidase H digestion, and also by treatment with brefeldin A. It was found that an N-glycosylation consensus site of the J-chain was functional, and intracellular J-chain was endoglycosidase H sensitive. These results indicate that, in the absence of any immunoglobulin molecules, J-chain localizes exclusively in the ER. We also tested whether the J-chain could be exported from the ER by perturbing the Ca2+ concentration in the ER. Cultivation of the J-chain transfectant in the presence of ionomycin resulted in the time-dependent secretion of the J-chain. The secreted J-chain was modified by the Golgi resident glycosylation enzymes, indicating that the secreted J-chain passed through the normal exocytic pathway.
Authors:
Masatake Asano; Yoshitaka Ogura; Nobuko Takenouchi-Ohkubo; Hirotsugu Chihaya; Wu Chung-Hsing; Kenji Ishikawa; Kunihiko Kobayashi; Jean-Pierre Vaerman; Itaru Moro
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  DNA and cell biology     Volume:  23     ISSN:  1044-5498     ISO Abbreviation:  DNA Cell Biol.     Publication Date:  2004 Jul 
Date Detail:
Created Date:  2004-08-05     Completed Date:  2004-08-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9004522     Medline TA:  DNA Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  403-11     Citation Subset:  IM    
Affiliation:
Department of Pathology, Nihon University School of Dentistry, Kanda Surugadai, Chiyoda-ku, Tokyo, 101-8310, Japan. asano-m@dent.nihon-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Brefeldin A / pharmacology
Calcium / metabolism*
Cells, Cultured
Cricetinae
Endoplasmic Reticulum / drug effects,  metabolism*
Glycosylation
Humans
Immunoglobulin J-Chains / drug effects,  genetics,  metabolism*
Ionomycin / pharmacology
Kidney / cytology,  drug effects,  metabolism
Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase / metabolism
Membrane Glycoproteins / genetics,  metabolism
Protein Transport / drug effects
Time Factors
Transfection
Tunicamycin / pharmacology
Viral Envelope Proteins / genetics,  metabolism
Chemical
Reg. No./Substance:
0/G protein, vesicular stomatitis virus; 0/Immunoglobulin J-Chains; 0/Membrane Glycoproteins; 0/Viral Envelope Proteins; 11089-65-9/Tunicamycin; 20350-15-6/Brefeldin A; 56092-81-0/Ionomycin; 7440-70-2/Calcium; EC 3.2.1.96/Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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