Document Detail


Endopeptidase EC 3.4.24.15 presence in the rat median eminence and hypophysial portal blood and its modulation of the luteinizing hormone surge.
MedLine Citation:
PMID:  9419832     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The endopeptidase EC 3.4.24.15 (EP24.15) is a zinc metalloendopeptidase that is widely distributed in a variety of tissues, including the testes, pituitary and the central nervous system. Among its numerous roles in metabolizing and processing biologically-active peptides, the enzyme degrades gonadotropin-releasing hormone (GnRH) by cleaving the central Tyr5-Gly6 bond. The aim of the present studies was to determine whether EP24.15 can modulate the concentrations of GnRH within the hypothalamo-hypophysial portal blood and thereby play a physiological role in reproduction. Our data suggest the presence of immunoreactive EP24.15 in the perivascular space of the median eminence and that this enzyme is secreted into portal blood. We have also shown a physiological role for this enzyme in that an inhibition of its activity with a specific inhibitor augmented the steroid-induced LH increase in ovariectomized rats. The present results suggest that secretory and post-secretory mechanisms are important in shaping the GnRH signal from the central nervous system; GnRH metabolism by EP24.15 may be one such mechanism.
Authors:
T J Wu; A R Pierotti; M Jakubowski; W J Sheward; M J Glucksman; A I Smith; J C King; G Fink; J L Roberts
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neuroendocrinology     Volume:  9     ISSN:  0953-8194     ISO Abbreviation:  J. Neuroendocrinol.     Publication Date:  1997 Nov 
Date Detail:
Created Date:  1998-01-30     Completed Date:  1998-01-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8913461     Medline TA:  J Neuroendocrinol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  813-22     Citation Subset:  IM    
Affiliation:
Fishberg Research Center for Neurobiology, Mount Sinai School of Medicine, New York, New York 10029, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Enzyme Inhibitors / pharmacology
Female
Gonadotropin-Releasing Hormone / metabolism
Immunohistochemistry
Luteinizing Hormone / secretion*
Male
Median Eminence / enzymology*
Metalloendopeptidases / analysis*,  antagonists & inhibitors,  blood*
Ovariectomy
Pituitary Gland / blood supply*
Portal System*
Rats
Rats, Sprague-Dawley
Chemical
Reg. No./Substance:
0/Enzyme Inhibitors; 33515-09-2/Gonadotropin-Releasing Hormone; 9002-67-9/Luteinizing Hormone; EC 3.4.24.-/Metalloendopeptidases; EC 3.4.24.15/thimet oligopeptidase

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