| Endonucleolytic activities of nuclei from rat ascites hepatoma. | |
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MedLine Citation:
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PMID: 3028420 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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By autodigestion (endogenous endonucleolysis) of rat liver (RL) or rat-ascites hepatoma (AH) nuclei, the nucleosomes were released from the RL, but not from the AH, nuclei. In contrast, by micrococcal nuclease digestion (exogenous endonucleolysis), the nucleosomes were released more rapidly from the AH than from the RL nuclei. A 0.6 M NaCl extract of the RL or AH nuclei was filtered through a Sephadex G-100 column. The resulting topoisomerase fraction was subjected to DNA relaxation and catenation assays with pBR322 DNA as a substrate. Consequently, the relaxation activity was almost the same between the RL and AH fractions, whereas the catenation activity was much higher in the AH fraction. |
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Authors:
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Y Hibino; N Sugano |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochemistry international Volume: 13 ISSN: 0158-5231 ISO Abbreviation: Biochem. Int. Publication Date: 1986 Nov |
Date Detail:
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Created Date: 1987-03-18 Completed Date: 1987-03-18 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8100311 Medline TA: Biochem Int Country: AUSTRALIA |
Other Details:
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Languages: eng Pagination: 773-9 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line Cell Nucleus / enzymology* DNA Topoisomerases, Type I / metabolism Endonucleases / metabolism* Kinetics Liver Neoplasms, Experimental / enzymology, ultrastructure* Micrococcal Nuclease Nucleosomes / ultrastructure* Rats |
| Chemical | |
Reg. No./Substance:
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0/Nucleosomes; EC 3.1.-/Endonucleases; EC 3.1.31.1/Micrococcal Nuclease; EC 5.99.1.2/DNA Topoisomerases, Type I |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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