Document Detail

Endonucleases that may recognize the ssDNA-backbone structure: purification from Sf9 cells and characterization.
MedLine Citation:
PMID:  12489994     Owner:  NLM     Status:  MEDLINE    
Endonucleases that cleave ssDNA under conditions that minimize the formation of secondary structures were detected in Spodoptera frugiperda Sf9 cells. One endonuclease was purified from Sf9 cells and another from Sf9 cells infected by baculoviruses. Polynucleotides containing an A- or T-tract, or a CAP binding region were digested in the presence of ATP at low Mg ions with these two nucleases. ATP could be replaced by citrate but not by EDTA. The cleavage patterns were different from those obtained with endonuclease I and exonuclease VII of Escherichia coli. The cleavages were dependent on the sequence of the polynucleotides but not associated with specific bases. EndoSfV cleaved mainly AT-rich regions.
Eiko Akaboshi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  DNA and cell biology     Volume:  21     ISSN:  1044-5498     ISO Abbreviation:  DNA Cell Biol.     Publication Date:  2002 Nov 
Date Detail:
Created Date:  2002-12-19     Completed Date:  2003-01-15     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  9004522     Medline TA:  DNA Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  839-45     Citation Subset:  IM    
Laboratory of Molecular Genetics, Institute for Molecular and Cellular Biology, Osaka University, 1-3 Yamadaoka, Suita 565-0871, Japan.
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MeSH Terms
Cell Line
Citric Acid / metabolism
DNA, Single-Stranded / metabolism*
Edetic Acid / metabolism
Endonucleases / isolation & purification,  metabolism*
Magnesium / metabolism
Spodoptera / enzymology
Substrate Specificity
Reg. No./Substance:
0/DNA, Single-Stranded; 60-00-4/Edetic Acid; 7439-95-4/Magnesium; 77-92-9/Citric Acid; EC 3.1.-/Endonucleases

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