Document Detail


Endonuclease-based logic gates and sensors using magnetic force-amplified readout of DNA scission on cantilevers.
MedLine Citation:
PMID:  16144415     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The endonuclease scission of magnetic particles functionalized with sequence-specific DNAs, which are associated on cantilevers, is followed by the magnetic force-amplified readout of the reactions by the nano-mechanical deflection/retraction of the cantilevers. The systems are employed to develop AND or OR logic gates and to detect single base mismatch specificity of the endonucleases. The two endonucleases EcoRI (E(A)) and AscI (E(B)) are used as inputs. The removal of magnetic particles linked to the cantilever by the duplexes 1/1a and 2/2a via the simultaneous cleavage of the DNAs by E(A) and E(B) leads to the retraction of the magnetically deflected cantilever and to the establishment of the "AND" gate. The removal of the magnetic particles linked to the cantilevers by the duplex 3/3a by either E(A) or E(B) leads to the retraction of the magnetically deflected cantilever and to the establishment of the "OR" gate. The magnetic force-amplified readout of endonuclease activities is also employed to reveal single base mismatch specificity of the biocatalysts.
Authors:
Yossi Weizmann; Roey Elnathan; Oleg Lioubashevski; Itamar Willner
Related Documents :
8445365 - Characterization of new baculovirus genotypes arising from inoculation of pieris brassi...
3032685 - Blii, a restriction endonuclease from bacillus licheniformis.
16896525 - Characterisation of the novel restriction endonuclease suii from sulfolobus islandicus.
291995 - Restriction endonuclease cleavage map and location of unique features of the dna of hep...
21155545 - Molecular dosimetry of n2-hydroxymethyl-dg dna adducts in rats exposed to formaldehyde.
6965455 - Mode of action of 9-beta-d-arabinosyladenine and 1-beta-d-arabinosylcytosine on dna syn...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of the American Chemical Society     Volume:  127     ISSN:  0002-7863     ISO Abbreviation:  J. Am. Chem. Soc.     Publication Date:  2005 Sep 
Date Detail:
Created Date:  2005-09-07     Completed Date:  2006-02-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7503056     Medline TA:  J Am Chem Soc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  12666-72     Citation Subset:  IM    
Affiliation:
Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Biosensing Techniques / instrumentation*,  methods
Computers, Molecular
DNA / chemistry*
Endonucleases / chemistry*
Magnetics*
Nanotechnology / instrumentation*
Sensitivity and Specificity
Chemical
Reg. No./Substance:
9007-49-2/DNA; EC 3.1.-/Endonucleases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Molecular recognition via triplex formation of mixed purine/pyrimidine DNA sequences using oligoTRIP...
Next Document:  Direct molecular detection of nucleic acids by fluorescence signal amplification.