Document Detail

Endonuclease-based logic gates and sensors using magnetic force-amplified readout of DNA scission on cantilevers.
MedLine Citation:
PMID:  16144415     Owner:  NLM     Status:  MEDLINE    
The endonuclease scission of magnetic particles functionalized with sequence-specific DNAs, which are associated on cantilevers, is followed by the magnetic force-amplified readout of the reactions by the nano-mechanical deflection/retraction of the cantilevers. The systems are employed to develop AND or OR logic gates and to detect single base mismatch specificity of the endonucleases. The two endonucleases EcoRI (E(A)) and AscI (E(B)) are used as inputs. The removal of magnetic particles linked to the cantilever by the duplexes 1/1a and 2/2a via the simultaneous cleavage of the DNAs by E(A) and E(B) leads to the retraction of the magnetically deflected cantilever and to the establishment of the "AND" gate. The removal of the magnetic particles linked to the cantilevers by the duplex 3/3a by either E(A) or E(B) leads to the retraction of the magnetically deflected cantilever and to the establishment of the "OR" gate. The magnetic force-amplified readout of endonuclease activities is also employed to reveal single base mismatch specificity of the biocatalysts.
Yossi Weizmann; Roey Elnathan; Oleg Lioubashevski; Itamar Willner
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of the American Chemical Society     Volume:  127     ISSN:  0002-7863     ISO Abbreviation:  J. Am. Chem. Soc.     Publication Date:  2005 Sep 
Date Detail:
Created Date:  2005-09-07     Completed Date:  2006-02-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7503056     Medline TA:  J Am Chem Soc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  12666-72     Citation Subset:  IM    
Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
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MeSH Terms
Biosensing Techniques / instrumentation*,  methods
Computers, Molecular
DNA / chemistry*
Endonucleases / chemistry*
Nanotechnology / instrumentation*
Sensitivity and Specificity
Reg. No./Substance:
9007-49-2/DNA; EC 3.1.-/Endonucleases

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