Document Detail


Endomyocardial biopsy derived adherent proliferating cells - a potential cell source for cardiac tissue engineering.
MedLine Citation:
PMID:  20013794     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Heart diseases are a leading cause of morbidity and mortality. Cardiac stem cells (CSC) are considered as candidates for cardiac-directed cell therapies. However, clinical translation is hampered since their isolation and expansion is complex. We describe a population of human cardiac derived adherent proliferating (CAP) cells that can be reliably and efficiently isolated and expanded from endomyocardial biopsies (0.1 cm(3)). Growth kinetics revealed a mean cell doubling time of 49.9 h and a high number of 2.54 x 10(7) cells in passage 3. Microarray analysis directed at investigating the gene expression profile of human CAP cells demonstrated the absence of the hematopoietic cell markers CD34 and CD45, and of CD90, which is expressed on mesenchymal stem cells (MSC) and fibroblasts. These data were confirmed by flow cytometry analysis. CAP cells could not be differentiated into adipocytes, osteoblasts, chondrocytes, or myoblasts, demonstrating the absence of multilineage potential. Moreover, despite the expression of heart muscle markers like alpha-sarcomeric actin and cardiac myosin, CAP cells cannot be differentiated into cardiomyocytes. Regarding functionality, CAP cells were especially positive for many genes involved in angiogenesis like angiopoietin-1, VEGF, KDR, and neuropilins. Globally, principal component and hierarchical clustering analysis and comparison with microarray data from many undifferentiated and differentiated reference cell types, revealed a unique identity of CAP cells. In conclusion, we have identified a unique cardiac tissue derived cell type that can be isolated and expanded from endomyocardial biopsies and which presents a potential cell source for cardiac repair. Results indicate that these cells rather support angiogenesis than cardiomyocyte differentiation.
Authors:
Marion Haag; Sophie Van Linthout; Sebastian E A Schr?der; Undine Freymann; Jochen Ringe; Carsten Tsch?pe; Michael Sittinger
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cellular biochemistry     Volume:  109     ISSN:  1097-4644     ISO Abbreviation:  J. Cell. Biochem.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-01-26     Completed Date:  2010-05-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8205768     Medline TA:  J Cell Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  564-75     Citation Subset:  IM    
Copyright Information:
(c) 2009 Wiley-Liss, Inc.
Affiliation:
Tissue Engineering Laboratory, Department of Rheumatology and Clinical Immunology, Charit?-Universit?tsmedizin Berlin, Tucholskystr. 2, 10117 Berlin, Germany. marion-renate.haag@charite.de
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Biopsy
Cell Differentiation
Cell Lineage
Cell Proliferation*
Female
Fluorescent Antibody Technique
Humans
Immunohistochemistry
Male
Mesenchymal Stem Cells / cytology,  metabolism
Middle Aged
Myocardium / cytology*,  pathology
Myocytes, Cardiac / cytology
Tissue Engineering / methods*

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