Document Detail

Endometrial response to sexual steroids as assessed by prostaglandin F(2alpha) output in explant culture and hormone receptor expression.
MedLine Citation:
PMID:  10895028     Owner:  NLM     Status:  MEDLINE    
The objective of this study was to evaluate the endometrial response to sexual steroids in organ culture using two means: prostaglandin (PG) F(2alpha) output in medium culture and steroid receptor immunoexpression in tissue. Human endometrium samples were classified in homogeneous and heterogeneous proliferative or secretory subsets. In proliferative endometrium explant culture, progesterone (10(-7) M) induced a significant decrease in PGF(2alpha) output, but this was not the case in secretory endometrium, whereas no significant effect of estradiol (10(-8) M) was observed. Before culture, homogeneous and heterogeneous proliferative endometrium presented the same pattern of estradiol receptor (ER) and progesterone receptor (PR) expression evaluated by quantitative immunocytochemistry. After culture, immunoreactive ER and PR were detected on the explant. PR immunoexpression rates after culture were lower than before culture in glands on homogeneous proliferative and in stroma on heterogeneous proliferative endometrium explants without in vitro steroid addition. In secretory endometrium, no significant difference was observed between ER or PR immunoexpression rates before culture and after culture. These results provided the hormonal receptivity status of endometrium after culture and will thus serve as a reference for evaluating in vitro steroid effects on endometrium explants. Our preliminary results suggest that cultures of endometrium explants are a valid model for studying the effects of hormonal treatment on homogeneous as well as heterogeneous endometrium. These data could be particularly relevant for evaluating the potential response to hormone stimulation and treatment of endometria sampled in perimenopausal patients.
S Illouz; L Boubli; M N Lavaut; C Allasia; C Charpin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Gynecologic and obstetric investigation     Volume:  50     ISSN:  0378-7346     ISO Abbreviation:  Gynecol. Obstet. Invest.     Publication Date:  2000  
Date Detail:
Created Date:  2000-08-17     Completed Date:  2000-08-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7900587     Medline TA:  Gynecol Obstet Invest     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  43-9     Citation Subset:  IM    
Copyright Information:
Copyright 2000 S. Karger AG, Basel
Pathology Department, School of Medicine, University of Aix-Marseille II, Marseille, France.
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MeSH Terms
Culture Techniques
Dinoprost / secretion*
Endometrium / chemistry,  drug effects*,  secretion
Estradiol / pharmacology
Gonadal Steroid Hormones / pharmacology*
Middle Aged
Progesterone / pharmacology
Receptors, Estradiol / analysis*
Receptors, Progesterone / analysis*
Reg. No./Substance:
0/Gonadal Steroid Hormones; 0/Receptors, Estradiol; 0/Receptors, Progesterone; 50-28-2/Estradiol; 551-11-1/Dinoprost; 57-83-0/Progesterone

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