Document Detail


Endometrial glands are required for preimplantation conceptus elongation and survival.
MedLine Citation:
PMID:  11369585     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Endometrial glands secrete molecules hypothesized to support conceptus growth and development. In sheep, endometrial gland morphogenesis occurs postnatally and can be epigenetically ablated by neonatal progestin exposure. The resulting stable adult uterine gland knockout (UGKO) phenotype was used here to test the hypothesis that endometrial glands are required for successful pregnancy. Mature UGKO ewes were bred repeatedly to fertile rams, but no pregnancies were detected by ultrasound on Day 25. Day 7 blastocysts from normal superovulated ewes were then transferred synchronously into Day 7 control or UGKO ewes. Ultrasonography on Days 25-65 postmating indicated that pregnancy was established in control, but not in UGKO ewes. To examine early uterine-embryo interactions, four control and eight UGKO ewes were bred to fertile rams. On Day 14, their uteri were flushed. The uterus of each control ewe contained two filamentous conceptuses of normal length. Uteri from four UGKO ewes contained no conceptus. Uteri of three UGKO ewes contained a single severely growth-retarded tubular conceptus, whereas the remaining ewe contained a single filamentous conceptus. Histological analyses of these uteri revealed that endometrial gland density was directly related to conceptus survival and developmental state. Day 14 UGKO uteri that were devoid of endometrial glands did not support normal conceptus development and contained either no conceptuses or growth-retarded tubular conceptuses. The Day 14 UGKO uterus with moderate gland development contained a filamentous conceptus. Collectively, these results demonstrate that endometrial glands and, by inference, their secretions are required for periimplantation conceptus survival and development.
Authors:
C A Gray; K M Taylor; W S Ramsey; J R Hill; F W Bazer; F F Bartol; T E Spencer
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biology of reproduction     Volume:  64     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2001 Jun 
Date Detail:
Created Date:  2001-05-22     Completed Date:  2001-08-02     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1608-13     Citation Subset:  IM    
Affiliation:
Center for Animal Biotechology and Genomics, Department of Animal Science, Texas A&M University, College Station, Texas 77843-2471, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blastocyst / physiology
Dinoprost / administration & dosage,  pharmacology
Drug Implants
Embryo Transfer
Embryonic Development*
Embryonic and Fetal Development*
Endometrium / anatomy & histology,  drug effects,  physiology*
Female
Interferon Type I / analysis
Pregnancy
Pregnancy Proteins / analysis
Pregnenediones / administration & dosage,  pharmacology
Progestins / administration & dosage,  pharmacology
Sheep / embryology*
Superovulation
Ultrasonography, Prenatal
Grant Support
ID/Acronym/Agency:
P30 ES09106/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
0/Drug Implants; 0/Interferon Type I; 0/Pregnancy Proteins; 0/Pregnenediones; 0/Progestins; 0/trophoblastin; 25092-41-5/norgestomet; 551-11-1/Dinoprost

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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