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Endometrial expression of progesterone induced blocking factor and galectins-1, -3, -9 and -3 binding protein in the luteal phase and early pregnancy in cattle.
MedLine Citation:
PMID:  21610087     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Progesterone induced blocking factor (PIBF) and galectins modulate the maternal immune response during pregnancy. We hypothesized that the relative transcript abundance of the above genes would be different during the luteal phase/early pregnancy, and would be affected by progesterone supplementation. To further test this hypothesis protein expression analyses were carried out to evaluate the abundance, and localization of LGALS9 and PIBF. Following oestrus synchronization, heifers were either inseminated (n=140) or not (n=70). Half of the heifers in each status (cyclic or potentially pregnant) were randomly assigned to receive a progesterone releasing intra-vaginal device (PRID) on Day 3 after oestrus, which elevated progesterone concentrations from Day 3.5 to 8 (p<0.05), resulting in four treatment groups: cyclic and pregnant heifers, each with normal and high progesterone. After confirmation of pregnancy status in inseminated animals, uterine tissue was collected on Days 5, 7, 13 or 16 of the luteal phase of the cycle/pregnancy. Gene and protein expression was determined using Q-RT-PCR and IHC, respectively, on 5 heifers per treatment per time point (i.e., 80 in total). Progesterone concentrations did not affect expression of any of the genes (p>0.05). LGALS9 and LGALS3BP were expressed at low levels in both cyclic and pregnant endometria until Day 13. On Day 16, expression increased only in the pregnant heifers (p<0.0001). LGALS1 and LGALS3 decreased on Day 7 (p<0.0001), and remained low until Day 16. Pregnancy had no effect on the expression of LGALS1, LGALS3 and PIBF. Additionally, LGALS9 and PIBF proteins were expressed in distinct uterine cell types. These results indicate that the galectins may be involved in uterine receptivity and/or implantation in heifers.
Authors:
Lilian A Okumu; Trudee Fair; Julia Szekeres-Bartho; Alan M O'Doherty; Mark A Crowe; James F Roche; Patrick Lonergan; Niamh Forde
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-5-24
Journal Detail:
Title:  Physiological genomics     Volume:  -     ISSN:  1531-2267     ISO Abbreviation:  -     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-5-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9815683     Medline TA:  Physiol Genomics     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
University College Dublin.
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