Document Detail


Endometrial cysteine-rich secretory protein 3 is inhibited by human chorionic gonadotrophin, and is increased in the decidua of tubal ectopic pregnancy.
MedLine Citation:
PMID:  19282327     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ectopic pregnancy (EP) remains a considerable cause of morbidity and occasional mortality. Currently, there is no reliable test to differentiate ectopic from intrauterine gestation. We have previously used array technology to demonstrate that differences in gene expression in decidualized endometrium from women with ectopic and intrauterine gestations could be used to identify candidate diagnostic biomarkers for EP. The aim of this study was to further investigate the decidual gene with the highest fold increase in EP, cysteine-rich secretory protein-3 (CRISP-3). Decidualized endometrium from gestation-matched women undergoing surgical termination of pregnancy (n = 8), evacuation of uterus for miscarriage (n = 6) and surgery for EP (n = 11) was subjected to quantitative RT-PCR, morphological assessment, immunohistochemistry and western blot analysis. Sera were analysed for progesterone and human chorionic gonadotrophin (hCG) levels. Immortalized endometrial epithelial cells were cultured with physiological concentrations of hCG. CRISP-3 mRNA and protein expression were greater in endometrium from ectopic when compared with intrauterine pregnancies (P < 0.05). CRISP-3 protein was localized to epithelium and granulocytes of endometrium. CRISP-3 serum concentrations were not different in women with ectopic compared with intrauterine pregnancies. CRISP-3 expression in endometrium was not related to the degree of decidualization or to serum progesterone levels. Endometrial CRISP-3 expression was inversely proportional to serum hCG concentrations (P < 0.001). Stimulation of endometrial epithelial cells with hCG in vitro caused a reduction in CRISP-3 expression (P < 0.01). The measurement of CRISP-3 in endometrium could provide an additional tool in the diagnosis of failing early pregnancy of unknown location. The absence of a local reduction in expression of CRISP-3 in decidualized endometrium of women with EP may be due to reduced exposure to hCG due to the ectopic location of the trophoblast.
Authors:
A W Horne; W C Duncan; A E King; S Burgess; P C Lourenco; P Cornes; P Ghazal; A R Williams; L Udby; H O D Critchley
Related Documents :
20728827 - Serous cystadenoma and ectopic pregnancy in the fallopian tube.
9715027 - Ectopic pregnancy--changing trends.
15181527 - Clinics in diagnostic imaging (98). heterotopic pregnancy with an intrauterine blighted...
3744127 - Problems of rudimentary horn pregnancy.
10592427 - Timing of subsequent pregnancy following treatment of molar pregnancy.
16167887 - Is the trophoblast an immune regulator? the role of toll-like receptors during pregnancy.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-03-12
Journal Detail:
Title:  Molecular human reproduction     Volume:  15     ISSN:  1460-2407     ISO Abbreviation:  Mol. Hum. Reprod.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-04-08     Completed Date:  2009-04-17     Revised Date:  2013-06-02    
Medline Journal Info:
Nlm Unique ID:  9513710     Medline TA:  Mol Hum Reprod     Country:  England    
Other Details:
Languages:  eng     Pagination:  287-94     Citation Subset:  IM    
Affiliation:
Division of Reproductive and Developmental Sciences, The University of Edinburgh, Edinburgh, UK. andrew.horne@ed.ac.uk
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Biological Markers / metabolism
Cell Line
Chorionic Gonadotropin / metabolism*
Decidua / cytology,  metabolism*,  pathology
Embryo Implantation
Endometrium / cytology,  metabolism,  pathology
Female
Humans
Microarray Analysis
Middle Aged
Pregnancy
Pregnancy, Ectopic / diagnosis,  metabolism*,  pathology
Progesterone / blood
Salivary Proteins and Peptides / antagonists & inhibitors*,  genetics,  metabolism*
Seminal Plasma Proteins / antagonists & inhibitors*,  genetics,  metabolism*
Trophoblasts / metabolism
Young Adult
Grant Support
ID/Acronym/Agency:
//Wellcome Trust
Chemical
Reg. No./Substance:
0/Biological Markers; 0/CRISP3 protein, human; 0/Chorionic Gonadotropin; 0/Salivary Proteins and Peptides; 0/Seminal Plasma Proteins; 57-83-0/Progesterone
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  GPR119 is required for physiological regulation of glucagon-like peptide-1 secretion but not for met...
Next Document:  Vinaxanthone, a new FabI inhibitor from Penicillium sp.