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Endomembrane dissection using chemically induced bioactive clusters.
MedLine Citation:
PMID:  24306872     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Chemical genomics is a novel approach that allows for the rapid functional analysis of plant proteins, complexes, pathways, and networks. Systematic screens for bioactive small molecules causing specific subcellular phenotypes have been successfully performed in mammalian cells, but thus far, are limited in plants. This protocol describes a systematic chemical screen of plasma membrane recycling markers in plants, using confocal microscopy and the subsequent clustering of subcellular phenotypes, to identify chemicals with desired effects. The method provides an approach to identify novel chemicals for pathway dissection, making chemical genomics more accessible to the scientific community. The matrix of novel chemicals described in this protocol can be expanded and analyzed continuously as more data is collected, increasing our knowledge of the endomembrane system, and accumulating compartment-specific markers and chemical probes that perturb specific aspects of endomembrane trafficking.
Authors:
Natasha Worden; Thomas Girke; Georgia Drakakaki
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  1056     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2014  
Date Detail:
Created Date:  2013-12-05     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  159-68     Citation Subset:  IM    
Affiliation:
Department of Plant Sciences, University of California, Davis, CA, USA.
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