Document Detail

Endogenous triacylglycerol metabolism in diabetic heart.
MedLine Citation:
PMID:  7091348     Owner:  NLM     Status:  MEDLINE    
The metabolism of endogenous triacylglycerols (TG) was studied in perfused working hearts of control and 12-day-old streptozotocin-treated diabetic rats. TG synthesis was assessed by the incorporation of exogenous [9,10(-3)H]palmitate. TG lipolysis was assessed by the following two methods: 1) measurement of the decrease in [14C]TG after prior in vivo isotopic prelabeling with [1-14C]palmitate and 2) calculation of the change in TG content as TG synthesis minus TG lipolysis. Control and diabetic hearts were perfused with buffer containing substrate concentrations characteristic of the in vivo state [normal: 9 mM glucose, 0.5 mM free fatty acid (FFA); diabetic: 27 mM glucose, 1.2 mM FFA]. Diabetic hearts were also perfused under normal substrate conditions. In diabetic hearts perfused under diabetic conditions elevated TG levels were maintained, lipolysis was reduced or unchanged (depending on the method of determination), and synthesis was enhanced. Oxidation of TG fatty acids (TGFA) was not impaired. Control hearts showed net lipolysis coupled with lower rates of exogenous FFA uptake and TG synthesis. In diabetic hearts perfused under normal substrate conditions lipolysis and TGFA oxidation were markedly enhanced. Thus we suggest that the basis of TG accumulation seen in the diabetic heart is due to both inhibition of lipolysis and enhancement of synthesis resulting from high levels of exogenous FFA and glucose.
D J Paulson; M F Crass
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The American journal of physiology     Volume:  242     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1982 Jun 
Date Detail:
Created Date:  1982-08-26     Completed Date:  1982-08-26     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  H1084-94     Citation Subset:  IM    
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MeSH Terms
Blood Pressure
Cholesterol / metabolism
Diabetes Mellitus, Experimental / metabolism*
Fatty Acids, Nonesterified / metabolism
Heart Rate
Myocardium / metabolism*
Phospholipids / metabolism
Rats, Inbred Strains
Triglycerides / metabolism*
Reg. No./Substance:
0/Fatty Acids, Nonesterified; 0/Phospholipids; 0/Triglycerides; 57-88-5/Cholesterol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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