| Endogenous protein S-Nitrosylation in E. coli: regulation by OxyR. | |
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MedLine Citation:
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PMID: 22539721 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Endogenous S-nitrosylation of proteins, a principal mechanism of cellular signaling in eukaryotes, has not been observed in microbes. We report that protein S-nitrosylation is an obligate concomitant of anaerobic respiration on nitrate in Escherichia coli. Endogenous S-nitrosylation during anaerobic respiration is controlled by the transcription factor OxyR, previously thought to operate only under aerobic conditions. Deletion of OxyR resulted in large increases in protein S-nitrosylation, and S-nitrosylation of OxyR induced transcription from a regulon that is distinct from the regulon induced by OxyR oxidation. Furthermore, products unique to the anaerobic regulon protected against S-nitrosothiols, and anaerobic growth of E. coli lacking OxyR was impaired on nitrate. Thus, OxyR serves as a master regulator of S-nitrosylation, and alternative posttranslational modifications of OxyR control distinct transcriptional responses. |
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Authors:
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Divya Seth; Alfred Hausladen; Ya-Juan Wang; Jonathan S Stamler |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Science (New York, N.Y.) Volume: 336 ISSN: 1095-9203 ISO Abbreviation: Science Publication Date: 2012 Apr |
Date Detail:
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Created Date: 2012-04-27 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0404511 Medline TA: Science Country: United States |
Other Details:
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Languages: eng Pagination: 470-3 Citation Subset: IM |
Affiliation:
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Institute for Transformative Molecular Medicine and Department of Medicine, Case Western Reserve University School of Medicine and University Hospitals Case Medical Center, Cleveland, OH 44106, USA. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GEO/GSE24524 |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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| Grant Support | |
ID/Acronym/Agency:
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R01-HL059130/HL/NHLBI NIH HHS; R01-HL095463/HL/NHLBI NIH HHS; R01-HL096673/HL/NHLBI NIH HHS |
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