| Endogenous prostaglandin formation in the field-stimulated guinea-pig vas deferens: comparison of the inhibitory effects of indomethacin and NS-398. | |
| | |
MedLine Citation:
|
PMID: 10718109 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Prostaglandin (PG) E2 inhibited both phases of contraction produced by electrical field stimulation of the guinea-pig vas deferens. PGF2alpha and PGD2 were without effect on this preparation. Carbacyclin (a PGI2) analogue inhibited the first phase of contraction at higher concentrations, whereas U46619 (a thromboxane mimetic) potentiated both phases of contraction. As exogenous arachidonic acid inhibits both phases of contraction of the electrically field-stimulated guinea-pig vas deferens, it is likely that the arachidonic acid is converted to PGE2 in the vas deferens. Indomethacin, a non-specific inhibitor of prostaglandin H synthase (PGHS), attenuated the inhibitory actions of exogenous arachidonic acid when examined on the first phase of contraction. NS-398, a relatively specific inhibitor of PGHS-2, also prevented the inhibitory action of exogenous arachidonic acid. However, NS-398 was much less effective than indomethacin in this respect even though NS-398 and indomethacin inhibit PGHS-2 with similar potencies. Consequently, the findings suggest that exogenous arachidonic acid is converted to PGE2 in the guinea-pig vas deferens by the actions of PGHS-1 and, to a lesser extent, by PGHS-2. |
| | |
Authors:
|
M R Miller; F C Johnson; N L Poyser |
Related Documents
:
|
1556389 - Nitrogen dioxide induced changes in level of free fatty acids, triglyceride, esterified... 9728089 - Ontogenetic and regional changes in alpha-methyl-d-glucoside and l-proline intestinal t... 6278679 - Biochemical studies in infective amosite pneumoconiosis. 1762679 - Fatty acids modulate excitability in guinea-pig hippocampal slices. 958649 - Food intake, dietary supplements and survival time of scorbutic guinea pigs. 5075269 - Stimulation of the production of unesterified fatty acids in nerve endings of guinea-pi... 3519869 - Extracellular overflow of neuroactive amino acids during severe insulin-induced hypogly... 18473739 - Alkylsquarates as key intermediates for the rapid preparation of original drug-inspired... 14572719 - Convenient preparation of l-arabino-hexos-5-ulose derivatives from lactose. |
Publication Detail:
|
Type: Comparative Study; Journal Article |
Journal Detail:
|
Title: Prostaglandins, leukotrienes, and essential fatty acids Volume: 61 ISSN: 0952-3278 ISO Abbreviation: Prostaglandins Leukot. Essent. Fatty Acids Publication Date: 1999 Dec |
Date Detail:
|
Created Date: 2000-04-03 Completed Date: 2000-04-03 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8802730 Medline TA: Prostaglandins Leukot Essent Fatty Acids Country: SCOTLAND |
Other Details:
|
Languages: eng Pagination: 363-8 Citation Subset: IM |
Affiliation:
|
Department of Pharmacology, University of Edinburgh Medical School, UK. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Arachidonic Acid / metabolism Cyclooxygenase Inhibitors / pharmacology Dinoprostone / metabolism Electric Stimulation Guinea Pigs Indomethacin / pharmacology Isoenzymes / drug effects Male Muscle Contraction / drug effects Nitrobenzenes / pharmacology Prostaglandin-Endoperoxide Synthases / drug effects* Prostaglandins / biosynthesis* Sulfonamides / pharmacology Vas Deferens / metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Cyclooxygenase Inhibitors; 0/Isoenzymes; 0/Nitrobenzenes; 0/Prostaglandins; 0/Sulfonamides; 123653-11-2/N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide; 363-24-6/Dinoprostone; 506-32-1/Arachidonic Acid; 53-86-1/Indomethacin; EC 1.14.99.1/Prostaglandin-Endoperoxide Synthases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The enzymes responsible for the metabolism of prostaglandins in bovine placenta.
Next Document: Vascular effects of isoprostanes after endothelial damage.