Document Detail

Endogenous production of nitric oxide contributes to proliferation effect of vascular endothelial growth factor-induced malignant melanoma cell.
MedLine Citation:
PMID:  16309495     Owner:  NLM     Status:  MEDLINE    
The objectives of this study were to observe the effect of overexpression of vascular endothelial growth factor (VEGF) on the proliferation of the malignant melanoma (MM) cell line A375, and to study the role of nitric oxide (NO) in this process and the mechanism of VEGF induced-A375 cell proliferation. The VEGF(165) cDNA was transfected into A375 cells by electroporation. VEGF mRNA and protein in A375 cells were detected by RT-PCR and ELISA. The proliferation of A375 cells was assessed by cell counting and MTT assay. Protein expression of iNOS, eNOS and nNOS was detected by Western blotting. NO production in A375 cell supernatant was measured by the nitrate reductase method. VEGF mRNA in A375 cells was significantly increased 72 h and 96 h after transfection of VEGF(165) cDNA, as were VEGF protein, NO and iNOS levels. However, protein expression of eNOS and nNOS was not detected in either transfected or untransfected cells. Proliferation of A375 cells transfected with VEGF(165) cDNA was enhanced. The nitric oxide synthase inhibitor l-NAME could dose-dependently inhibit the proliferation of A375 cells evoked by VEGF. These results indicate that VEGF enhances the expression of iNOS in A375 cells and results in an increase in NO formation, which may be important in the process of VEGF-induced proliferation of A375 cells.
J Tao; Y-T Tu; J-W Li; A-P Feng; C-Z Huang; Y Wu; G-X Shen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical and experimental dermatology     Volume:  31     ISSN:  0307-6938     ISO Abbreviation:  Clin. Exp. Dermatol.     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2005-11-28     Completed Date:  2006-05-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7606847     Medline TA:  Clin Exp Dermatol     Country:  England    
Other Details:
Languages:  eng     Pagination:  94-9     Citation Subset:  IM    
Department of Dermatology, Affiliated Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
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MeSH Terms
Cell Division / drug effects,  physiology
Cell Line, Tumor
Enzyme Inhibitors / pharmacology
Melanoma, Experimental / physiopathology*
NG-Nitroarginine Methyl Ester / pharmacology
Nitric Oxide / biosynthesis*
Nitric Oxide Synthase Type I / analysis
Nitric Oxide Synthase Type II / analysis
Nitric Oxide Synthase Type III / analysis
Nitrites / analysis
RNA, Messenger / analysis
RNA, Neoplasm / analysis
Vascular Endothelial Growth Factors / physiology*
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Nitrites; 0/RNA, Messenger; 0/RNA, Neoplasm; 0/Vascular Endothelial Growth Factors; 10102-43-9/Nitric Oxide; 50903-99-6/NG-Nitroarginine Methyl Ester; EC Oxide Synthase Type I; EC Oxide Synthase Type II; EC Oxide Synthase Type III

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