Document Detail


Endogenous neurotrophic factors enhance neurite growth by bag cell neurons of Aplysia.
MedLine Citation:
PMID:  12767034     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mechanisms that regulate neurite outgrowth are phylogenetically conserved, including the signaling molecules involved. Here, we describe neurotrophic effects on isolated bag cell neurons (BCNs) of substrate-bound growth factors endogenous to the sea slug Aplysia californica. Sheath cells dissociated from the pleural-visceral connectives of the Aplysia CNS and arterial cells dissociated from the anterior aorta enhance neurite outgrowth when compared to controls, i.e., BCNs grown in defined medium alone. In addition, the substrate remaining after sheath cells or arterial cells are killed significantly enhances growth, relative to all other conditions tested. For instance, primary neurites are more numerous and greater in length for BCNs cultured on substrate produced by arterial cells. These results suggest that sheath and arterial cells produce growth-promoting factors, some of which are found in the substrates produced by these cell types. Using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), we found that Aplysia collagen-like peptides are produced by dissociated arterial cells, and therefore likely contribute to the observed growth effects. Collagen-like peptides and other factors produced by sheath and arterial cells likely influence neurite growth in the Aplysia CNS during development, learning and memory, and regeneration after injury.
Authors:
Binaben H Vanmali; Elena V Romanova; Maria C Messner; Mona Singh; Joel Maruniak; Jonathan Sweedler; Mark D Kirk
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of neurobiology     Volume:  56     ISSN:  0022-3034     ISO Abbreviation:  J. Neurobiol.     Publication Date:  2003 Jul 
Date Detail:
Created Date:  2003-05-26     Completed Date:  2003-07-24     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0213640     Medline TA:  J Neurobiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  78-93     Citation Subset:  IM    
Copyright Information:
Copyright 2003 Wiley Periodicals, Inc. J Neurobiol 56: 78-93, 2003
Affiliation:
Division of Biological Sciences, University of Missouri-Columbia, 101 Lefevre Hall, Columbia, Missouri 65211, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Aorta / chemistry,  cytology*,  physiology
Aplysia / cytology,  physiology*
Carrier Proteins*
Cells, Cultured
Central Nervous System / chemistry,  cytology*,  physiology
Coculture Techniques
Image Processing, Computer-Assisted
Immunohistochemistry
Nerve Growth Factors / physiology*
Neurites / physiology*,  ultrastructure
Neuropeptides / metabolism
Peptides / analysis
Proteins / metabolism
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Grant Support
ID/Acronym/Agency:
6R01 NS 31609/NS/NINDS NIH HHS; RR10249/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/Nerve Growth Factors; 0/Neuropeptides; 0/Peptides; 0/Proteins; 0/c1q-binding proteins; 115857-46-0/histidine rich basic peptide (Aplysia)

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