Document Detail


Endogenous methyl palmitate modulates nicotinic receptor-mediated transmission in the superior cervical ganglion.
MedLine Citation:
PMID:  19057014     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nitric oxide (NO) is identified as the endothelium-derived relaxing factor and a neurotransmitter with a superfusion bioassay cascade technique. By using a similar technique with rat superior cervical ganglion (SCG) as donor tissue and rabbit endothelium-denuded aortic ring as detector tissue, we report here that a vasodilator, which is more potent than NO, is released in the SCG upon field electrical stimulation (FES) or addition of nicotine. Release of this vasodilator was enhanced by arginine analogs, including N(omega)-nitro-l-arginine (a NO synthase inhibitor), suggesting that it is not NO. Analysis by gas chromatography/mass spectrometry identified 2 saturated fatty acids, palmitic acid methyl ester (PAME) and stearic acid methyl ester (SAME), being released from the SCG upon FES in the presence of arginine analogs. Exogenous PAME but not SAME induced significant aortic dilation (EC(50) = 0.19 nM), indicating that PAME is the potent vasodilator. Release of PAME and SAME was significantly diminished in chronically decentralized SCG but not denervated SCG, suggesting the preganglionic origin. Furthermore, release of both fatty acids was calcium- and myosin light chain kinase-dependent, suggesting that both were released from axoplasmic vesicular stores. Electrophysiological studies further demonstrated that PAME but not SAME inhibited nicotine-induced inward currents in cultured SCG and the alpha7-nicotinic acetylcholine receptor-expressing Xenopus oocytes. Endogenous PAME appears to play a role in modulation of the autonomic ganglionic transmission and to complement the vasodilator effect of NO.
Authors:
Hung Wen Lin; Chao-Zong Liu; Deshou Cao; Po-Yi Chen; Mei-Fang Chen; Shinn-Zong Lin; Mansoor Mozayan; Alex F Chen; Louis S Premkumar; Donald S Torry; Tony J-F Lee
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2008-12-04
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  105     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-12-10     Completed Date:  2009-01-30     Revised Date:  2013-06-04    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  19526-31     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, Southern Illinois University School of Medicine, Springfield, IL 62702, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Electric Stimulation
Enzyme Inhibitors / pharmacology
Male
Nicotine / pharmacology
Nicotinic Agonists / pharmacology
Nitric Oxide / metabolism
Nitroarginine / pharmacology
Oocytes / physiology
Palmitates / metabolism*
Patch-Clamp Techniques
Rats
Rats, Sprague-Dawley
Receptors, Nicotinic / metabolism*
Stearic Acids / metabolism
Superior Cervical Ganglion / cytology,  drug effects,  metabolism*
Sympathectomy
Synaptic Transmission / drug effects,  physiology*
Vasodilation / physiology
Xenopus
Grant Support
ID/Acronym/Agency:
HL 27763/HL/NHLBI NIH HHS; HL 47574/HL/NHLBI NIH HHS; R01 HD036830-07/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Nicotinic Agonists; 0/Palmitates; 0/Receptors, Nicotinic; 0/Stearic Acids; 0/alpha7 nicotinic acetylcholine receptor; 10102-43-9/Nitric Oxide; 2149-70-4/Nitroarginine; 54-11-5/Nicotine; DPY8VCM98I/methyl palmitate
Comments/Corrections
Erratum In:
Proc Natl Acad Sci U S A. 2009 Mar 10;106(10):4060

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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