Document Detail


Endogenous loss of leucine and methionine in adult male rats.
MedLine Citation:
PMID:  857877     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. The fractional rate of loss of 14C and body-weight was measured in adult male rats after giving 14C-labelled methionine or leucine and maintaining rats for 30 d on a low-protein or a specific methionine+cystine-free diet: carcasses were then analysed for protein and fat 14C radioactivity. 2. The fractional loss of 14CO2 from [14C]methionine or [14C]leucine between day 20 and day 30 was always greater than the fractional loss of body-weight. 3. Carcass protein 14C radioactivity after giving [14C]leucine was higher than after giving [14C]methionine, but fat 14C radioactivity after either 14C-labelled amino acid was only a small proportion of the total body 14C radioactivity. 4. After correction of the fractional loss of 14CO2 for urinary 14C loss, but not body-weight loss, absolute amino acid loss was calculated using published values for methionine and leucine content of rats. 5. The best estimates of endogenous amino acid loss obtained using I-14C-labelled amino acids, expressed as mg/kg body-weight 0.75 per day were leucine 79, methionine 38.
Authors:
R J Neale; J C Waterlow
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The British journal of nutrition     Volume:  37     ISSN:  0007-1145     ISO Abbreviation:  Br. J. Nutr.     Publication Date:  1977 Mar 
Date Detail:
Created Date:  1977-06-30     Completed Date:  1977-06-30     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0372547     Medline TA:  Br J Nutr     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  259-68     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Body Composition
Body Weight
Carbon Dioxide / metabolism
Diet
Leucine / metabolism*
Male
Methionine / metabolism*
Nutritional Physiological Phenomena*
Proteins / metabolism
Rats
Time Factors
Chemical
Reg. No./Substance:
0/Proteins; 124-38-9/Carbon Dioxide; 61-90-5/Leucine; 63-68-3/Methionine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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