Document Detail


Endogenous inhibitor of nonlysosomal high molecular weight protease and calcium-dependent protease.
MedLine Citation:
PMID:  3020549     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An endogenous inhibitor of high molecular weight protease was purified from human erythrocytes and partially characterized. The inhibitor was isolated by DEAE-Sephacel ion-exchange chromatography followed by separation on a Bio-Gel A-0.5m column. The inhibitor displayed a native Mr of 240,000 and contained a single subunit of Mr 40,000 after NaDodSO4/polyacrylamide gel electrophoresis. The Mr 240,000 hexamer inhibited high molecular weight protease noncompetitively (Ki = 8.3 X 10(-8) M) and showed marked susceptibility to proteolytic digestion and heat treatment. The purified factor was also a potent inhibitor of calcium-dependent protease (Ki = 2.8 X 10(-8) M), whereas it had no effect on trypsin, chymotrypsin, or papain. Heat treatment (50-70 degrees C X 10 min) caused loss of inhibition against high molecular weight protease; however, inhibition of calcium-dependent protease was stable under the same conditions. This result is consistent with different domains on the inhibitor that interact with high molecular weight protease and calcium-dependent protease. Together with earlier studies in which repression of inhibitor by an ATP-ubiquitin-dependent process was proposed, the present results suggest a general mechanism for regulation of multiple nonlysosomal proteases that are complexed with endogenous inhibitors.
Authors:
K Murakami; J D Etlinger
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  83     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1986 Oct 
Date Detail:
Created Date:  1986-11-18     Completed Date:  1986-11-18     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7588-92     Citation Subset:  IM; S    
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / pharmacology
Animals
Calpain / antagonists & inhibitors*
Humans
Molecular Weight
Protease Inhibitors / analysis,  isolation & purification*,  pharmacology
Rabbits
Grant Support
ID/Acronym/Agency:
1R01 HL 31494/HL/NHLBI NIH HHS; IT 32 AM 07289/AM/NIADDK NIH HHS
Chemical
Reg. No./Substance:
0/Protease Inhibitors; 56-65-5/Adenosine Triphosphate; EC 3.4.22.-/Calpain
Comments/Corrections

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