Document Detail

Endogenous generation of cyanide in neuronal tissue: involvement of a peroxidase system.
MedLine Citation:
PMID:  10956427     Owner:  NLM     Status:  MEDLINE    
In a study of the mechanism by which cyanide is produced in neural tissue, it was hypothesized that nerve cells generate cyanide in a manner similar to that in leukocytes. As in white blood cells, glycine addition enhanced cyanide production in rat pheochromocytoma cells. Because myeloperoxidase catalyses cyanide production in leukocytes, a selective myeloperoxidase inhibitor (aminobenzoic acid hydrazide) was tested and found to inhibit opiate agonist-induced cyanide production in pheochromocytoma cells and also in rat brain. In addition, hydrogen peroxide enhanced cyanide release in pheochromocytoma cells, further suggesting that the process is oxidative in nature. Sonicated rat pheochromocytoma cells did not generate cyanide in response to an agonist acting on surface receptors even though disrupted cells responded to glycine. The mitochondrial fraction from rat brain produced more cyanide in response to glycine than any other fraction. Thus glycine seems to act at an intracellular site to enhance cyanide production and the process seems to involve a peroxidase mechanism similar to that reported for white blood cells.
P G Gunasekar; J L Borowitz; J J Turek; D A Van Horn; G E Isom
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of neuroscience research     Volume:  61     ISSN:  0360-4012     ISO Abbreviation:  J. Neurosci. Res.     Publication Date:  2000 Sep 
Date Detail:
Created Date:  2000-09-07     Completed Date:  2000-11-30     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7600111     Medline TA:  J Neurosci Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  570-5     Citation Subset:  IM    
Copyright Information:
Copyright 2000 Wiley-Liss, Inc.
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, Indiana, USA.
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MeSH Terms
Aminobenzoic Acids / pharmacology
Analgesics, Opioid / pharmacology
Azides / pharmacology
Brain Chemistry / drug effects
Carbachol / pharmacology
Cell Fractionation
Cholinergic Agonists / pharmacology
Cyanides / metabolism*
Dose-Response Relationship, Drug
Glycine / pharmacology
Hydrogen Peroxide / pharmacology
Hydromorphone / pharmacology
Mitochondria / drug effects,  metabolism
Morphine / pharmacology
Narcotics / pharmacology
Neurons / cytology,  drug effects,  metabolism*
Oxidants / pharmacology
PC12 Cells
Peroxidases / antagonists & inhibitors,  metabolism*
Rats, Sprague-Dawley
Grant Support
Reg. No./Substance:
0/Aminobenzoic Acids; 0/Analgesics, Opioid; 0/Azides; 0/Cholinergic Agonists; 0/Cyanides; 0/Narcotics; 0/Oxidants; 466-99-9/Hydromorphone; 51-83-2/Carbachol; 56-40-6/Glycine; 57-27-2/Morphine; 7722-84-1/Hydrogen Peroxide; EC 1.11.1.-/Peroxidases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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