| Endogenous generation of cyanide in neuronal tissue: involvement of a peroxidase system. | |
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MedLine Citation:
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PMID: 10956427 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In a study of the mechanism by which cyanide is produced in neural tissue, it was hypothesized that nerve cells generate cyanide in a manner similar to that in leukocytes. As in white blood cells, glycine addition enhanced cyanide production in rat pheochromocytoma cells. Because myeloperoxidase catalyses cyanide production in leukocytes, a selective myeloperoxidase inhibitor (aminobenzoic acid hydrazide) was tested and found to inhibit opiate agonist-induced cyanide production in pheochromocytoma cells and also in rat brain. In addition, hydrogen peroxide enhanced cyanide release in pheochromocytoma cells, further suggesting that the process is oxidative in nature. Sonicated rat pheochromocytoma cells did not generate cyanide in response to an agonist acting on surface receptors even though disrupted cells responded to glycine. The mitochondrial fraction from rat brain produced more cyanide in response to glycine than any other fraction. Thus glycine seems to act at an intracellular site to enhance cyanide production and the process seems to involve a peroxidase mechanism similar to that reported for white blood cells. |
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Authors:
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P G Gunasekar; J L Borowitz; J J Turek; D A Van Horn; G E Isom |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of neuroscience research Volume: 61 ISSN: 0360-4012 ISO Abbreviation: J. Neurosci. Res. Publication Date: 2000 Sep |
Date Detail:
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Created Date: 2000-09-07 Completed Date: 2000-11-30 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7600111 Medline TA: J Neurosci Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 570-5 Citation Subset: IM |
Copyright Information:
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Copyright 2000 Wiley-Liss, Inc. |
Affiliation:
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Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, Indiana, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aminobenzoic Acids
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pharmacology Analgesics, Opioid / pharmacology Animals Azides / pharmacology Brain Chemistry / drug effects Carbachol / pharmacology Cell Fractionation Cholinergic Agonists / pharmacology Cyanides / metabolism* Dose-Response Relationship, Drug Glycine / pharmacology Hydrogen Peroxide / pharmacology Hydromorphone / pharmacology Male Mitochondria / drug effects, metabolism Morphine / pharmacology Narcotics / pharmacology Neurons / cytology, drug effects, metabolism* Oxidants / pharmacology PC12 Cells Peroxidases / antagonists & inhibitors, metabolism* Rats Rats, Sprague-Dawley |
| Grant Support | |
ID/Acronym/Agency:
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ES04140/ES/NIEHS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Aminobenzoic Acids; 0/Analgesics, Opioid; 0/Azides; 0/Cholinergic Agonists; 0/Cyanides; 0/Narcotics; 0/Oxidants; 466-99-9/Hydromorphone; 51-83-2/Carbachol; 56-40-6/Glycine; 57-27-2/Morphine; 7722-84-1/Hydrogen Peroxide; EC 1.11.1.-/Peroxidases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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