Document Detail

Endogenous butyrylcholinesterase in SV40 transformed cell lines: COS-1, COS-7, MRC-5 SV40, and WI-38 VA13.
MedLine Citation:
PMID:  7842168     Owner:  NLM     Status:  MEDLINE    
Comparison of proteins expressed by SV40 transformed cell lines and untransformed cell lines is of interest because SV40 transformed cells are immortal, whereas untransformed cells senesce after about 50 doublings. In MRC-5 SV40 cells, only seven proteins have previously been reported to shift from undetectable to detectable after transformation by SV40 virus. We report that butyrylcholinesterase is an 8th protein in this category. Butyrylcholinesterase activity in transformed MRC-5 SV40 cells increased at least 150-fold over its undetectable level in MRC-5 parental cells. Other SV40 transformed cell lines, including COS-1, COS-7, and WI-38 VA13, also expressed endogenous butyrylcholinesterase, whereas the parental, untransformed cell lines, CV-1 and WI-38, had no detectable butyrylcholinesterase activity or mRNA. Infection of CV-1 cells by SV40 virus did not result in expression of butyrylcholinesterase, showing that the butyrylcholinesterase promoter was not activated by the large T antigen of SV40. We conclude that butyrylcholinesterase expression resulted from events related to cell immortalization and did not result from activation by the large T antigen.
M Kris; O Jbilo; C F Bartels; P Masson; S Rhode; O Lockridge
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  In vitro cellular & developmental biology. Animal     Volume:  30A     ISSN:  1071-2690     ISO Abbreviation:  In Vitro Cell. Dev. Biol. Anim.     Publication Date:  1994 Oct 
Date Detail:
Created Date:  1995-03-07     Completed Date:  1995-03-07     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9418515     Medline TA:  In Vitro Cell Dev Biol Anim     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  680-9     Citation Subset:  IM    
Eppley Institute, University of Nebraska Medical Center, Omaha 68198.
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MeSH Terms
Acetylcholinesterase / genetics,  metabolism
Blotting, Northern
Butyrylcholinesterase / genetics,  metabolism*
Carcinoma, Hepatocellular / enzymology
Cell Line, Transformed
Cell Transformation, Viral*
Cercopithecus aethiops
Embryo, Mammalian
Immunosorbent Techniques
Liver Neoplasms / enzymology
RNA, Messenger / metabolism
Simian virus 40*
Tumor Cells, Cultured
Grant Support
Reg. No./Substance:
0/RNA, Messenger; EC 3.1.1.-/Butyrylcholinesterase; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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