Document Detail


Endogenous biotin as a marker of adrenocortical cells with steroidogenic potential.
MedLine Citation:
PMID:  21256921     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Interpretation of adrenal cortex phenotypes is greatly facilitated by simultaneous examination of multiple markers at single cell resolution. However, the availability of multiple appropriate antibodies can be rate limiting, while their cognate antigens are often subject to variable accessibility. Specific markers not subject to these constraints thus have obvious utility. Here we report that endogenous biotin, when detected in fixed, frozen tissue sections using fluorescent streptavidin, is a specific marker of apparently all cells with steroidogenic potential in the murine adrenal cortex. While streptavidin stains presteroidogenic and mature cortical cells, it does not label the adrenal capsule, medulla or vascular endothelium. Developmental profiles reveal adrenal endogenous biotin labeling from E13.5 through adulthood. Comparisons with zonal markers, hypothalamic-pituitary-adrenal (HPA) axis-remodeled tissue, transgenic Shh-nLacZ or Gli1-nLacZ animals, and Shh mutant embryos further demonstrate the utility of this approach. Fluorescent streptavidin applied using a simple one-step staining protocol thus provides a potent counterstain for use in adrenal analyses.
Authors:
Alex Paul; Ed Laufer
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-01-21
Journal Detail:
Title:  Molecular and cellular endocrinology     Volume:  336     ISSN:  1872-8057     ISO Abbreviation:  Mol. Cell. Endocrinol.     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-03-07     Completed Date:  2011-06-22     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  7500844     Medline TA:  Mol Cell Endocrinol     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  133-40     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.
Affiliation:
Department of Pathology and Cell Biology, Columbia University Medical Center, New York, NY 10032, USA. ajp2142@columbia.edu
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MeSH Terms
Descriptor/Qualifier:
Adrenal Cortex / cytology*,  growth & development,  metabolism*
Animals
Animals, Newborn
Biological Markers / metabolism
Biotin / metabolism*
Cryoultramicrotomy
Hedgehog Proteins / metabolism
Mice
Signal Transduction
Staining and Labeling
Steroids / biosynthesis*
Streptavidin / metabolism
Time Factors
Tissue Fixation
Grant Support
ID/Acronym/Agency:
R01 DK 08515/DK/NIDDK NIH HHS; R01 DK081515-02/DK/NIDDK NIH HHS; R01 DK081515-03/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Hedgehog Proteins; 0/Shh protein, mouse; 0/Steroids; 58-85-5/Biotin; 9013-20-1/Streptavidin
Comments/Corrections

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