| Endogenous biotin as a marker of adrenocortical cells with steroidogenic potential. | |
| | |
MedLine Citation:
|
PMID: 21256921 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Interpretation of adrenal cortex phenotypes is greatly facilitated by simultaneous examination of multiple markers at single cell resolution. However, the availability of multiple appropriate antibodies can be rate limiting, while their cognate antigens are often subject to variable accessibility. Specific markers not subject to these constraints thus have obvious utility. Here we report that endogenous biotin, when detected in fixed, frozen tissue sections using fluorescent streptavidin, is a specific marker of apparently all cells with steroidogenic potential in the murine adrenal cortex. While streptavidin stains presteroidogenic and mature cortical cells, it does not label the adrenal capsule, medulla or vascular endothelium. Developmental profiles reveal adrenal endogenous biotin labeling from E13.5 through adulthood. Comparisons with zonal markers, hypothalamic-pituitary-adrenal (HPA) axis-remodeled tissue, transgenic Shh-nLacZ or Gli1-nLacZ animals, and Shh mutant embryos further demonstrate the utility of this approach. Fluorescent streptavidin applied using a simple one-step staining protocol thus provides a potent counterstain for use in adrenal analyses. |
| | |
Authors:
|
Alex Paul; Ed Laufer |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-01-21 |
Journal Detail:
|
Title: Molecular and cellular endocrinology Volume: 336 ISSN: 1872-8057 ISO Abbreviation: Mol. Cell. Endocrinol. Publication Date: 2011 Apr |
Date Detail:
|
Created Date: 2011-03-07 Completed Date: 2011-06-22 Revised Date: 2012-09-24 |
Medline Journal Info:
|
Nlm Unique ID: 7500844 Medline TA: Mol Cell Endocrinol Country: Ireland |
Other Details:
|
Languages: eng Pagination: 133-40 Citation Subset: IM |
Copyright Information:
|
Copyright © 2011 Elsevier Ireland Ltd. All rights reserved. |
Affiliation:
|
Department of Pathology and Cell Biology, Columbia University Medical Center, New York, NY 10032, USA. ajp2142@columbia.edu |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adrenal Cortex
/
cytology*,
growth & development,
metabolism* Animals Animals, Newborn Biological Markers / metabolism Biotin / metabolism* Cryoultramicrotomy Hedgehog Proteins / metabolism Mice Signal Transduction Staining and Labeling Steroids / biosynthesis* Streptavidin / metabolism Time Factors Tissue Fixation |
| Grant Support | |
ID/Acronym/Agency:
|
R01 DK 08515/DK/NIDDK NIH HHS; R01 DK081515-02/DK/NIDDK NIH HHS; R01 DK081515-03/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Biological Markers; 0/Hedgehog Proteins; 0/Shh protein, mouse; 0/Steroids; 58-85-5/Biotin; 9013-20-1/Streptavidin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Hypospadias: Interactions between environment and genetics.
Next Document: Serum brain-derived neurotrophic factor and clozapine daily dose in patients with schizophrenia: A p...