Document Detail


Endogenous transcription at the centromere facilitates centromere activity in budding yeast.
MedLine Citation:
PMID:  22000103     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The centromere (CEN) DNA-kinetochore complex is the specialized chromatin structure that mediates chromosome attachment to the spindle and is required for high-fidelity chromosome segregation. Although kinetochore function is conserved from budding yeast to humans, it was thought that transcription had no role in centromere function in budding yeast, in contrast to other eukaryotes including fission yeast.
RESULTS: We report here that transcription at the centromere facilitates centromere activity in the budding yeast Saccharomyces cerevisiae. We identified transcripts at CEN DNA and found that Cbf1, which is a transcription factor that binds to CEN DNA, is required for transcription at CEN DNA. Chromosome instability of cbf1Δ cells is suppressed by transcription driven from an artificial promoter. Furthermore, we have identified Ste12, which is a transcription factor, and Dig1, a Ste12 inhibitor, as a novel CEN-associated protein complex by an in vitro kinetochore assembly system. Dig1 represses Ste12-dependent transcription at the centromere.
CONCLUSIONS: Our studies reveal that transcription at the centromere plays an important role in centromere function in budding yeast.
Authors:
Kentaro Ohkuni; Katsumi Kitagawa
Related Documents :
14534903 - Siliceous spicules and skeleton frameworks in sponges: origin, diversity, ultrastructur...
1618023 - Orientation and segregation of a micromanipulated multivalent: familiar principles, div...
21693773 - Dynlt3 is required for chromosome alignment during mouse oocyte meiotic maturation.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-10-13
Journal Detail:
Title:  Current biology : CB     Volume:  21     ISSN:  1879-0445     ISO Abbreviation:  Curr. Biol.     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-10-28     Completed Date:  2012-08-13     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  9107782     Medline TA:  Curr Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1695-703     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Ltd. All rights reserved.
Affiliation:
Center for Childhood Cancer, The Research Institute, Nationwide Children's Hospital, Columbus, OH 43205, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / genetics,  metabolism
Centromere / genetics*,  metabolism*
Gene Expression Regulation, Fungal
Kinetochores / metabolism
Promoter Regions, Genetic
Saccharomyces cerevisiae / genetics*
Saccharomyces cerevisiae Proteins / genetics,  metabolism*
Transcription Factors / genetics,  metabolism
Transcription, Genetic
Grant Support
ID/Acronym/Agency:
CA21765/CA/NCI NIH HHS; GM68418/GM/NIGMS NIH HHS; R01 GM068418/GM/NIGMS NIH HHS; R01 GM068418-06A2/GM/NIGMS NIH HHS; R01 GM068418-07/GM/NIGMS NIH HHS; R01 GM068418-08/GM/NIGMS NIH HHS; R01 GM068418-09/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; 0/CBF1 protein, S cerevisiae; 0/DIG1 protein, S cerevisiae; 0/STE12 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins; 0/Transcription Factors
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Adaptive Secondary Sex Ratio Adjustments via Sex-Specific Infanticide in a Bird.
Next Document:  Nonretinotopic exogenous attention.