Document Detail

Endogenous N-losses in broilers estimated by a [15N]-isotope dilution technique: effect of dietary fat type and xylanase addition.
MedLine Citation:
PMID:  10836259     Owner:  NLM     Status:  MEDLINE    
Male broilers were given a low protein diet (15.5% CP) spiked with [15N]H4HCO3 from day 12 to day 18 of age to label the endogenous N-constituents. Experimental diets were subsequently fed from day 19 to day 24 of age and consisted of a rye based diet (56% dietary inclusion) which contained either 10% soya oil (S) or 10% beef tallow (T), each of which was either unsupplemented (-) or supplemented (+) with a xylanase containing enzyme preparation (2700 IU/kg at pH 5.3). [15N]-atom percent excess (APE) of excreta, faeces and urine were monitored on a daily basis during both experimental periods. Furthermore, APE was measured in various tissues at the end of the experiment. The APE of urine on the last day of the experiment was between the APE of the pancreas and that of the jejunal tissue, an observation which supported the usefulness of using urinary APE as an indicator for the endogenous N-pool. Endogenous N-proportions were estimated by an isotope dilution technique at the end of the experiment by examination of the ratio of APE in faeces and urine. The endogenous N-proportion in the faeces was greatest in birds receiving the T(-) diet. The proportions were 0.321, 0.319, 0.451 and 0.289 in S(-), S(+), T(-) and T(+) fed groups, respectively. Xylanase addition reduced endogenous N-proportion, a factor which was used to correct apparent crude protein digestibility (85.6, 86.2, 84.3 and 88.5% in S(-), S(+), T(-) and T(+) fed birds, respectively) for endogenous losses resulting in almost equal true digestibilities of crude protein for all treatments (90.3, 90.6, 90.4 and 91.5%). The amounts of endogenous N in faces were estimated to be 87, 69, 244 and 81 mg per day per kg0.67 body weight in S(-), S(+), T(-) and T(+) fed birds, respectively. It was concluded that xylanase supplementation of a rye based broiler diet does not change endogenous N-secretions when the supplemental fat is soya oil. However, addition of tallow rather than soya oil increased these N-losses significantly, an effect which was reversed by xylanase addition.
S Dänicke; H Jeroch; O Simon
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archiv für Tierernährung     Volume:  53     ISSN:  0003-942X     ISO Abbreviation:  Arch Tierernahr     Publication Date:  2000  
Date Detail:
Created Date:  2000-06-20     Completed Date:  2000-06-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217641     Medline TA:  Arch Tierernahr     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  75-97     Citation Subset:  IM    
Institut für Tierernährung und Vorratshaltung, Landwirtschaftliche Fakultät, Martin-Luther-Universität Halle-Wittenberg, Halle (Saale), Germany.
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MeSH Terms
Animal Feed
Chickens / metabolism*
Dietary Fats / metabolism*
Fatty Acids / metabolism
Feces / chemistry
Gas Chromatography-Mass Spectrometry / veterinary
Indicator Dilution Techniques / veterinary
Jejunum / chemistry
Nitrogen / analysis,  metabolism*,  urine
Nitrogen Isotopes / analysis
Secale cereale / metabolism
Soybean Oil / metabolism
Uric Acid / analysis
Xylan Endo-1,3-beta-Xylosidase
Xylosidases / metabolism*
Reg. No./Substance:
0/Dietary Fats; 0/Fatty Acids; 0/Nitrogen Isotopes; 69-93-2/Uric Acid; 7727-37-9/Nitrogen; 8001-22-7/Soybean Oil; EC 3.2.1.-/Xylosidases; EC Endo-1,3-beta-Xylosidase

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