| Endodermal differentiation of murine embryonic carcinoma cells by retinoic acid requires JLP, a JNK-scaffolding protein. | |
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MedLine Citation:
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PMID: 16619266 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Retinoic acid (RA) is a morphogen that induces endodermal differentiation of murine P19 embryonic carcinoma cells. RA-induced differentiation of P19 cells has been used as a model system to define the differentiation programs of pluripotent stem cells. Using this system it has been shown that G alpha13--the alpha-subunit of the heterotrimeric G protein G13--and its activation of JNK-module are critically required for the endodermal differentiation of P19 cells. However, the mechanism through which G alpha13 is linked to JNK-module is unknown. Here, we report that RA stimulates the expression of JNK-interacting leucine zipper protein (JLP), a newly identified JNK-scaffolding protein and its critical role in RA-mediated endodermal differentiation. Our results indicate that there is a physical association between JLP and G alpha13 in RA-stimulated P19 cells. More interestingly, silencing JLP abrogates RA-mediated endodermal differentiation of P19 cells analogous to the effects seen with the silencing of G alpha13 or JNK. Therefore, our studies presented here identify for the first time, a novel role for a newly identified scaffolding protein in RA-mediated endodermal differentiation, providing a new signaling conduit to transmit signals from RA to JNK module. |
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Authors:
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Kimia Kashef; Hua Xu; E Premkumar Reddy; Danny N Dhanasekaran |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Journal of cellular biochemistry Volume: 98 ISSN: 0730-2312 ISO Abbreviation: J. Cell. Biochem. Publication Date: 2006 Jul |
Date Detail:
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Created Date: 2006-06-29 Completed Date: 2006-08-16 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 8205768 Medline TA: J Cell Biochem Country: United States |
Other Details:
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Languages: eng Pagination: 715-22 Citation Subset: IM |
Copyright Information:
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2006 Wiley-Liss, Inc. |
Affiliation:
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Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adaptor Proteins, Signal Transducing
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metabolism* Animals Antineoplastic Agents / pharmacology* Carcinoma, Embryonal / metabolism* Cell Differentiation / drug effects* Cell Line, Tumor Endoderm / metabolism* GTP-Binding Protein alpha Subunits, G12-G13 / metabolism Mice Models, Biological* Pluripotent Stem Cells / metabolism* Signal Transduction / drug effects Tretinoin / pharmacology* |
| Grant Support | |
ID/Acronym/Agency:
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AG22022/AG/NIA NIH HHS; GM49897/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Adaptor Proteins, Signal Transducing; 0/Antineoplastic Agents; 0/Spag9 protein, mouse; 302-79-4/Tretinoin; EC 3.6.5.1/GTP-Binding Protein alpha Subunits, G12-G13 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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