| Endocytic uptake pathways utilized by CPMV nanoparticles. | |
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MedLine Citation:
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PMID: 22905759 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Cowpea mosaic virus (CPMV) has been used as a nanoparticle platform for biomedical applications including vaccine development, in-vivo vascular imaging, and tissue-targeted delivery. A better understanding of the mechanisms of CPMV targeting and cell internalization would enable enhanced targeting and more effective delivery. Previous studies showed that, following binding and internalization by mammalian cells, CPMV localizes in a perinuclear late-endosome compartment where it remains for as long as several days. To further investigate endocytic trafficking of CPMV within the cell, we used multiple approaches including pharmacologic inhibition of pathways, and co-localization with endocytic vesicle compartments. CPMV internalization was clathrin-independent, and utilized a combination of caveolar endocytosis and macropinocytosis pathways for entry. CPMV particles co-localized with Rab5+ early endosomes to traffic ultimately to a lysosomal compartment. These studies facilitate the further development of effective intracellular drug-delivery strategies using CPMV. |
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Authors:
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Emily Megan Plummer; Marianne Manchester |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-8-20 |
Journal Detail:
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Title: Molecular pharmaceutics Volume: - ISSN: 1543-8392 ISO Abbreviation: Mol. Pharm. Publication Date: 2012 Aug |
Date Detail:
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Created Date: 2012-8-21 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101197791 Medline TA: Mol Pharm Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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