Document Detail


Endocytic uptake pathways utilized by CPMV nanoparticles.
MedLine Citation:
PMID:  22905759     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cowpea mosaic virus (CPMV) has been used as a nanoparticle platform for biomedical applications including vaccine development, in vivo vascular imaging, and tissue-targeted delivery. A better understanding of the mechanisms of CPMV targeting and cell internalization would enable enhanced targeting and more effective delivery. Previous studies showed that, following binding and internalization by mammalian cells, CPMV localizes in a perinuclear late-endosome compartment where it remains for as long as several days. To further investigate endocytic trafficking of CPMV within the cell, we used multiple approaches including pharmacologic inhibition of pathways and colocalization with endocytic vesicle compartments. CPMV internalization was clathrin-independent and utilized a combination of caveolar endocytosis and macropinocytosis pathways for entry. CPMV particles colocalized with Rab5(+) early endosomes to traffic ultimately to a lysosomal compartment. These studies facilitate the further development of effective intracellular drug-delivery strategies using CPMV.
Authors:
Emily M Plummer; Marianne Manchester
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-09-20
Journal Detail:
Title:  Molecular pharmaceutics     Volume:  10     ISSN:  1543-8392     ISO Abbreviation:  Mol. Pharm.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-08     Completed Date:  2013-09-19     Revised Date:  2014-01-16    
Medline Journal Info:
Nlm Unique ID:  101197791     Medline TA:  Mol Pharm     Country:  United States    
Other Details:
Languages:  eng     Pagination:  26-32     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Transport
Cells, Cultured
Comovirus / metabolism*
Drug Delivery Systems / methods
Endocytosis / physiology*
Endosomes / metabolism,  physiology,  virology
HeLa Cells
Humans
Macrophages / metabolism,  virology
Mice
Nanoparticles / administration & dosage*
Pinocytosis / physiology
Grant Support
ID/Acronym/Agency:
P30 NS047101/NS/NINDS NIH HHS; R01 CA112075/CA/NCI NIH HHS; R01CA112075/CA/NCI NIH HHS
Comments/Corrections

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