| Endocytic routes to the Golgi apparatus. | |
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MedLine Citation:
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PMID: 9681635 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The endocytic routes of labelled lectins as well as cationic ferritin were studied in cells with a regulated secretion, i.e. pancreatic beta cells, and in constitutively secreting cells, i.e. fibroblasts and HepG2 hepatoma cells, paying particular attention to routes into the Golgi apparatus. Considerable amounts of internalised molecules were taken up into the trans Golgi network (TGN) and into Golgi subcompartments in all three cell types as well as in secretory granules of the pancreatic beta cells. The internalised materials did not pass rapidly the TGN and Golgi stacks, but were still present hours after internalisation, being then particularly concentrated in TGN-elements and in the transmost Golgi cisterna. Endocytosed materials reached forming secretory granules present in the TGN. Further, direct fusion between endocytotic vesicles and mature secretory granules was observed. Golgi subcompartments as well as endocytic TGN containing endocytosed materials were in close apposition to specialised regions of the endoplasmic reticulum. The Golgi apparatus including its parts containing endocytosed materials were transformed into a tubular reticulum upon treatment with the fungal metabolite Brefeldin A. Rarely, internalised material was observed in the lumen of the endoplasmic reticulum, thus providing evidence for an endocytic plasma membrane to endoplasmic reticulum route. |
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Authors:
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M Pavelka; A Ellinger; P Debbage; C Loewe; M Vetterlein; J Roth |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Histochemistry and cell biology Volume: 109 ISSN: 0948-6143 ISO Abbreviation: Histochem. Cell Biol. Publication Date: 1998 May-Jun |
Date Detail:
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Created Date: 1998-10-15 Completed Date: 1998-10-15 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9506663 Medline TA: Histochem Cell Biol Country: GERMANY |
Other Details:
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Languages: eng Pagination: 555-70 Citation Subset: IM |
Affiliation:
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Institute of Histology and Embryology, University of Innsbruck, Austria. margit.pavelka@uibk.ac.at |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Transport Cell Compartmentation Cells, Cultured Endoplasmic Reticulum / ultrastructure Ferritins Fibroblasts / ultrastructure Fluorescent Antibody Technique, Indirect Golgi Apparatus / ultrastructure* Humans Islets of Langerhans / ultrastructure Lectins Liver Neoplasms, Experimental / pathology Microscopy, Fluorescence Rats |
| Chemical | |
Reg. No./Substance:
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0/Lectins; 9007-73-2/Ferritins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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