Document Detail

Endocytic Uptake of FITC-Albumin by Human Alveolar Epithelial Cell Line A549.
MedLine Citation:
PMID:  22214936     Owner:  NLM     Status:  Publisher    
The uptake mechanism of FITC-labeled albumin (FITC-albumin) was examined in human alveolar epithelial cell line A549. FITC-albumin uptake by A549 cells was time- and temperature-dependent, and was markedly suppressed at 4°C compared with that at 37°C. The uptake was saturable, and was mediated by a high-affinity, low capacity system and by a low-affinity, high capacity system. In the following experiments, we focused on the low-affinity system. FITC-albumin uptake was markedly inhibited by metabolic inhibitors and by a vacuolar H(+)-ATPase, bafilomycin A(1). The uptake was inhibited by clathrin-mediated endocytosis inhibitors (phenylarsine oxide and chlorpromazine). Potassium depletion and hypertonicity that inhibit clathrin-mediated endocytosis also decreased FITC-albumin uptake. On the other hand, caveolae-mediated endocytosis inhibitors (indomethacin and nystatin) did not affect FITC-albumin uptake. In addition, FITC-albumin uptake was inhibited by macropinocytosis inhibitors such as 5-(N-ethyl-N-isopropyl) amiloride. These results suggest that the low-affinity system of FITC-albumin uptake is mediated by endocytosis in A549 cells, predominantly via a clathrin-mediated pathway. Macropinocytosis, but not caveolae-mediated endocytosis, may also be involved. Considering our previous findings, albumin may be transported by the similar mechanism and/or pathway in rat and human alveolar epithelial cells.
Ryoko Yumoto; Sayuri Suzuka; Keisuke Oda; Junya Nagai; Mikihisa Takano
Related Documents :
10537196 - Morphogenesis is coordinated with nuclear division in germinating aspergillus nidulans ...
3501266 - Triradial configurations indicate that expression of the fragile site at xq27 is non-le...
19747476 - Grandparental stem cells in leech segmentation: differences in cdc42 expression are cor...
21451586 - Nitrogen fixation and transfer in open ocean diatom-cyanobacterial symbioses.
2998356 - Epidermal growth factor receptors in the human glioblastoma cell line sf268 differ from...
12942086 - Sphingosylphosphorylcholine regulates keratin network architecture and visco-elastic pr...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-3
Journal Detail:
Title:  Drug metabolism and pharmacokinetics     Volume:  -     ISSN:  1880-0920     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-1-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101164773     Medline TA:  Drug Metab Pharmacokinet     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of Pharmaceutics and Therapeutics, Graduate School of Biomedical Sciences, Hiroshima University.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Phase and electrical behaviour in Bi(4)NbO(8.5).
Next Document:  The Impact of Pharmacogenomics Research on Drug Development.