Document Detail

Endocrine and mucous differentiation by a cloned human rectal adenocarcinoma cell line (HRA-19) in vitro: inhibition by TGF-beta 1.
MedLine Citation:
PMID:  8056828     Owner:  NLM     Status:  MEDLINE    
Colorectal epithelium is composed of absorptive, mucous and endocrine cells, all of which are considered to arise from a common stem cell located in the crypt base. However, the factors controlling the commitment to differentiate are poorly understood. This is partly due to the lack of in vitro model systems for the study of differentiation in colorectal epithelium. The HRA-19 cell line, established from a human rectal adenocarcinoma, has been shown to have multipotential characteristics with cloned HRA-19 cells able to differentiate into absorptive, mucous and endocrine cells when grown as xenografts. The lack of such differentiated cells in HRA-19 monolayers in vitro suggests that differentiation is controlled by extracellular matrix, stromal cells and/or soluble factors. Such observations show that differentiation in HRA-19 cells can be controlled by extrinsic factors and therefore provide a model system for studying control of differentiation in colorectal epithelium. Unfortunately, the restriction of differentiation to xenografts of the cell line limits the degree to which this differentiation can be manipulated. In this study, the possibility that HRA-19 cells could be induced to differentiate in vitro under appropriate conditions has been investigated. Endocrine and mucous cells were identified by immunocytochemistry with differentiation-related antibodies and histology of monolayers. Preconfluent HRA-19 cells grown in 10% foetal calf serum formed a well polarised monolayer with apical tight junctions and sparse microvilli, but cells with mucous or endocrine phenotypes were only very occasionally observed. However, endocrine and mucous cells could reproducibly be demonstrated in postconfluent monolayers grown in 1% foetal calf serum.(ABSTRACT TRUNCATED AT 250 WORDS)
S C Kirkland; K Henderson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of cell science     Volume:  107 ( Pt 4)     ISSN:  0021-9533     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  1994 Apr 
Date Detail:
Created Date:  1994-09-13     Completed Date:  1994-09-13     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1041-6     Citation Subset:  IM    
Department of Histopathology, Royal Postgraduate Medical School, London, UK.
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MeSH Terms
Adenocarcinoma / pathology*
Cell Differentiation / drug effects
Endocrine Glands / drug effects,  pathology*
Intestinal Mucosa / drug effects,  pathology*
Neoplastic Stem Cells / drug effects,  pathology*
Rectal Neoplasms / pathology*
Transforming Growth Factor beta / pharmacology*
Tumor Cells, Cultured
Reg. No./Substance:
0/Transforming Growth Factor beta

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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