| Endocrine and cellular characteristics of corpora lutea from cows with a delayed post-ovulatory progesterone rise. | |
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MedLine Citation:
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PMID: 16289469 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The timing of the post-ovulatory progesterone rise is critical to the embryonic development and survival. The aim of this study was to determine the underlying causes of delayed post-ovulatory progesterone rises. Two groups of non-lactating dairy cows with early (n = 11) or late (n = 9) post-ovulatory progesterone rises were created by inducing luteolysis in the presence of either a large (> 10 mm) or small (< 10 mm) follicle, respectively. LH pulses were measured on days 4 (all cows) and 7 (n = 7, early; n = 5, late) (day 1= ovulation). The cows were slaughtered on day 5 (n = 4 each group) or 8 (n = 7, early; n = 5, late). Immunohistochemical analysis for endothelial cells (von Willebrand Factor, VWF), steroidogenic cells (3beta-HSD) and proliferation marker (Ki67) were performed. The basal progesterone production and LH responsiveness (0.001-100 ng/ml) of dispersed luteal cells was investigated. The luteal concentrations of FGF-2 and VEGF were measured by ELISA and RIA, respectively. There were no differences in LH pulse characteristics, area of VWF staining, proliferation index, steroidogenic cell characteristics, basal or LH-stimulated progesterone production by luteal cells between cows with an early or late progesterone rise (P > 0.10). However, the area of VWF staining increased from days 5 to 8, while the proliferation index decreased (P < 0.05). Furthermore, the luteal cells were more responsive to LH on day 8 (P < 0.01). Luteal concentrations of FGF-2 were higher on day 5 (P = 0.05), while VEGF was greater on day 8 (P < 0.01). In conclusion, we have clearly shown that LH support, degree of vascularization or luteal cell steroidogenic capacity were not the major factors responsible for inadequate secretion of progesterone by the developing bovine CL. |
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Authors:
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R S Robinson; A J Hammond; L T Nicklin; D Schams; G E Mann; M G Hunter |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-10-25 |
Journal Detail:
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Title: Domestic animal endocrinology Volume: 31 ISSN: 0739-7240 ISO Abbreviation: Domest. Anim. Endocrinol. Publication Date: 2006 Aug |
Date Detail:
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Created Date: 2006-06-07 Completed Date: 2006-08-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8505191 Medline TA: Domest Anim Endocrinol Country: United States |
Other Details:
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Languages: eng Pagination: 154-72 Citation Subset: IM |
Affiliation:
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Division of Animal Physiology, University of Nottingham, Sutton Bonington Campus, Loughborough, Leics LE12 5RD, UK. Bob.Robinson@nottingham.ac.uk |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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metabolism Animals Blotting, Western / veterinary Cattle / physiology* Cell Proliferation Corpus Luteum / blood supply, cytology, metabolism, physiology* Female Fibroblast Growth Factor 2 / metabolism Hydroxysteroid Dehydrogenases / metabolism Immunohistochemistry / veterinary Ki-67 Antigen / metabolism Linear Models Luteal Cells / cytology, physiology Luteinizing Hormone / blood Neovascularization, Physiologic / physiology Ovulation Induction / veterinary Progesterone / blood, physiology* Random Allocation Vascular Endothelial Growth Factor A / metabolism von Willebrand Factor / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Ki-67 Antigen; 0/Vascular Endothelial Growth Factor A; 0/von Willebrand Factor; 103107-01-3/Fibroblast Growth Factor 2; 57-83-0/Progesterone; 9002-67-9/Luteinizing Hormone; EC 1.1.-/Hydroxysteroid Dehydrogenases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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