Document Detail

Endocrine and cellular characteristics of corpora lutea from cows with a delayed post-ovulatory progesterone rise.
MedLine Citation:
PMID:  16289469     Owner:  NLM     Status:  MEDLINE    
The timing of the post-ovulatory progesterone rise is critical to the embryonic development and survival. The aim of this study was to determine the underlying causes of delayed post-ovulatory progesterone rises. Two groups of non-lactating dairy cows with early (n = 11) or late (n = 9) post-ovulatory progesterone rises were created by inducing luteolysis in the presence of either a large (> 10 mm) or small (< 10 mm) follicle, respectively. LH pulses were measured on days 4 (all cows) and 7 (n = 7, early; n = 5, late) (day 1= ovulation). The cows were slaughtered on day 5 (n = 4 each group) or 8 (n = 7, early; n = 5, late). Immunohistochemical analysis for endothelial cells (von Willebrand Factor, VWF), steroidogenic cells (3beta-HSD) and proliferation marker (Ki67) were performed. The basal progesterone production and LH responsiveness (0.001-100 ng/ml) of dispersed luteal cells was investigated. The luteal concentrations of FGF-2 and VEGF were measured by ELISA and RIA, respectively. There were no differences in LH pulse characteristics, area of VWF staining, proliferation index, steroidogenic cell characteristics, basal or LH-stimulated progesterone production by luteal cells between cows with an early or late progesterone rise (P > 0.10). However, the area of VWF staining increased from days 5 to 8, while the proliferation index decreased (P < 0.05). Furthermore, the luteal cells were more responsive to LH on day 8 (P < 0.01). Luteal concentrations of FGF-2 were higher on day 5 (P = 0.05), while VEGF was greater on day 8 (P < 0.01). In conclusion, we have clearly shown that LH support, degree of vascularization or luteal cell steroidogenic capacity were not the major factors responsible for inadequate secretion of progesterone by the developing bovine CL.
R S Robinson; A J Hammond; L T Nicklin; D Schams; G E Mann; M G Hunter
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-10-25
Journal Detail:
Title:  Domestic animal endocrinology     Volume:  31     ISSN:  0739-7240     ISO Abbreviation:  Domest. Anim. Endocrinol.     Publication Date:  2006 Aug 
Date Detail:
Created Date:  2006-06-07     Completed Date:  2006-08-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8505191     Medline TA:  Domest Anim Endocrinol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  154-72     Citation Subset:  IM    
Division of Animal Physiology, University of Nottingham, Sutton Bonington Campus, Loughborough, Leics LE12 5RD, UK.
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MeSH Terms
Actins / metabolism
Blotting, Western / veterinary
Cattle / physiology*
Cell Proliferation
Corpus Luteum / blood supply,  cytology,  metabolism,  physiology*
Fibroblast Growth Factor 2 / metabolism
Hydroxysteroid Dehydrogenases / metabolism
Immunohistochemistry / veterinary
Ki-67 Antigen / metabolism
Linear Models
Luteal Cells / cytology,  physiology
Luteinizing Hormone / blood
Neovascularization, Physiologic / physiology
Ovulation Induction / veterinary
Progesterone / blood,  physiology*
Random Allocation
Vascular Endothelial Growth Factor A / metabolism
von Willebrand Factor / metabolism
Reg. No./Substance:
0/Actins; 0/Ki-67 Antigen; 0/Vascular Endothelial Growth Factor A; 0/von Willebrand Factor; 103107-01-3/Fibroblast Growth Factor 2; 57-83-0/Progesterone; 9002-67-9/Luteinizing Hormone; EC 1.1.-/Hydroxysteroid Dehydrogenases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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