Document Detail

Endocardiogenesis in embryoid bodies: novel markers identified by gene expression profiling.
MedLine Citation:
PMID:  17462595     Owner:  NLM     Status:  MEDLINE    
Endocardial cells and cardiomyocytes differentiate from the cardiogenic mesoderm at about the same time during development. Although in vitro embryonic stem (ES) cell systems have been used to study the differentiation of various types of cell lineages, including cardiomyocytes, smooth muscle cells, and vascular endothelial cells, differentiation of endocardial cells, or endocardiogenesis, has not been well reported, because of a lack of specific molecular markers. In our search for cardiogenesis-associated genes expressed in embryoid bodies, we found several genes expressed in the heart region of mouse embryos, but not in cardiomyocytes. To identify the cell types expressing these genes, CD31(+) cells were taken from mouse embryos on embryonic day (E)8.5 and E9.5 and sorted, then their transcripts were analyzed using quantitative RT-PCR analyses. In those embryos, Gata4 and Nfatc1, as well as newly identified Cgnl1 and Dok4 were found to be preferentially expressed in endocardial cells, but not in yolk sac endothelial cells, while Cdh5 and Kdr were expressed in both cardiac and yolk sac endothelial cells. Immunohistochemical analyses of embryoid bodies revealed that some CD31(+) cells co-expressing Gata4 and Nfatc1 were located in close proximity to cardiomyocytes. These results suggest that embryoid bodies express endocardial specific genes and likely generate endocardial cells along with cardiomyocytes. Further, they indicate that these new marker genes are useful to study the origin and induction of endocardial cells, and identify other endocardial markers.
Hiromichi Narumiya; Kyoko Hidaka; Manabu Shirai; Hiromi Terami; Hiroyuki Aburatani; Takayuki Morisaki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-04-17
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  357     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-05-14     Completed Date:  2007-07-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  896-902     Citation Subset:  IM    
Department of Bioscience, National Cardiovascular Center Research Institute, 5-7-1 Fujishirodai, Suita, Osaka 565-8565, Japan.
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MeSH Terms
Biological Markers
Cell Differentiation
Cells, Cultured
Embryonic Stem Cells / cytology*,  physiology*
Endocardium / cytology,  growth & development*,  metabolism*
GATA4 Transcription Factor / metabolism*
Gene Expression Profiling / methods*
Gene Expression Regulation, Developmental / physiology
NFATC Transcription Factors / metabolism*
Reg. No./Substance:
0/Biological Markers; 0/GATA4 Transcription Factor; 0/Gata4 protein, mouse; 0/NFATC Transcription Factors; 0/Nfatc1 protein, mouse

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