Document Detail


Endocannabinoids are implicated in the infarct size-reducing effect conferred by heat stress preconditioning in isolated rat hearts.
MedLine Citation:
PMID:  12160959     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: We have investigated the involvement of the endocannabinoid system in the delayed cardioprotection conferred by heat stress preconditioning in the isolated rat heart. METHODS: Rats were divided into eight groups (n=7 in each group), subjected to either heat stress (42 degrees C for 15 min, HS groups) or sham anaesthesia (Sham groups). Twenty-four hours later, their hearts were isolated, retrogradely perfused, and subjected to a 30-min occlusion of the left coronary artery followed by 120 min of reperfusion. Some hearts were perfused with either SR 141716 (a cannabinoid CB(1) receptor antagonist, 1 microM), SR 144528 (a CB(2) receptor antagonist, 1 microM) or L-NAME (a NOS inhibitor, 3 microM) 5 min before ischaemia and during the ischaemic period. RESULTS: The infarct size-reducing effect conferred by heat stress (35.7+/-1.8% in Sham to 14.1+/-0.6% in HS groups) was not altered by the perfusion of SR 141716 (11.2+/-1.5%) but was abolished by both SR 144528 (36.6+/-1.6%) and L-NAME (32.0+/-4.4%). In hearts from non-heat-stressed rats, perfusion with SR 141716 (32.8+/-1.6%), SR 144528 (33.4+/-2.2%) and L-NAME (31.6+/-2.9%) had no effect on infarct size. CONCLUSION: These results suggest an involvement of endocannabinoids, acting through CB(2) receptors, and NO in the cardioprotection conferred by heat stress against myocardial ischaemia. The possible interaction between both mediators of the heat stress response remains to be determined.
Authors:
Marie Joyeux; Claire Arnaud; Diane Godin-Ribuot; Pierre Demenge; Daniel Lamontagne; Christophe Ribuot
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Cardiovascular research     Volume:  55     ISSN:  0008-6363     ISO Abbreviation:  Cardiovasc. Res.     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-08-05     Completed Date:  2002-11-05     Revised Date:  2009-09-04    
Medline Journal Info:
Nlm Unique ID:  0077427     Medline TA:  Cardiovasc Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  619-25     Citation Subset:  IM    
Affiliation:
Laboratoire Stress Cardiovasculaires et Pathologies Associées, Faculté de Pharmacie, Domaine de la Merci, 38706 La Tronche, France. marie.joyeux@ujf-grenoble.fr
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MeSH Terms
Descriptor/Qualifier:
Analysis of Variance
Animals
Bornanes / pharmacology
Cannabinoids / antagonists & inhibitors,  metabolism*
Endocannabinoids
Fever / metabolism*,  pathology
Ischemic Preconditioning, Myocardial / methods*
Male
Myocardial Infarction / pathology,  prevention & control*
Myocardium / metabolism*,  pathology
NG-Nitroarginine Methyl Ester / pharmacology
Nitric Oxide Synthase / antagonists & inhibitors
Perfusion
Piperidines / pharmacology
Pyrazoles / pharmacology
Rats
Rats, Wistar
Receptor, Cannabinoid, CB2*
Receptors, Cannabinoid
Receptors, Drug / drug effects
Time Factors
Chemical
Reg. No./Substance:
0/Bornanes; 0/Cannabinoids; 0/Cnr2 protein, rat; 0/Endocannabinoids; 0/Piperidines; 0/Pyrazoles; 0/Receptor, Cannabinoid, CB2; 0/Receptors, Cannabinoid; 0/Receptors, Drug; 0/SR 144528; 158681-13-1/rimonabant; 50903-99-6/NG-Nitroarginine Methyl Ester; EC 1.14.13.39/Nitric Oxide Synthase

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