| Endocannabinoid metabolomics: a novel liquid chromatography-mass spectrometry reagent for fatty acid analysis. | |
| | |
MedLine Citation:
|
PMID: 17233529 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We have synthesized 4,4-dimethoxyoxazoline derivatives of several fatty acids associated with the endocannabinoid metabolome using tris(hydroxymethyl)aminomethane in a 1-step reaction by microwave irradiation. The derivatization incorporates a nitrogen into the final product, which allows for improved detection by liquid chromatography-mass spectrometry in positive atmospheric pressure chemical ionization (APCI) mode. Palmitic and oleic acid derivatives show a 200-fold increase in sensitivity compared with the free acids when analyzed in negative-mode APCI. In addition to improving sensitivity, the oxazoline derivatization creates a similar ionization response for the fatty acids tested, which simplifies their quantitation. Fatty acid oxazoline derivatives can be detected using the same conditions optimized for the endocannabinoids, which allows for a simultaneous quantitation of the entire endocannabinoid metabolome. |
| | |
Authors:
|
John Williams; Lakshmipathi Pandarinathan; JodiAnne Wood; Paul Vouros; Alexandros Makriyannis |
Related Documents
:
|
19171409 - Synthesis and evaluation of a novel series of heterocyclic oleanolic acid derivatives w... 15999339 - Insecticidal and fungicidal activity of new synthesized chitosan derivatives. 7483759 - Oral immunoadjuvant activity of lipophilic derivatives of n-acetylglucosaminyl-beta(1--... 18568909 - Enhancement in skin permeation of 5-aminolevulinic acid using l-menthol and its derivat... 20108029 - 6,7-difluoro-1,4-dihydro-1-methyl-4-oxo-3-quinolinecarboxylic acid, a newly designed fl... 15368569 - An approach towards the quantitative structure-activity relationships of caffeic acid a... 14646179 - Screening chemical composition and in vitro antioxidant and antimicrobial activities of... 21121249 - Cytotoxic evaluation of semisynthetic ester and amide derivatives of oleanolic acid. 381199 - Unique lipid composition of treponema pallidum (nichols virulent strain). |
Publication Detail:
|
Type: Journal Article Date: 2006-10-20 |
Journal Detail:
|
Title: The AAPS journal Volume: 8 ISSN: 1550-7416 ISO Abbreviation: AAPS J Publication Date: 2006 |
Date Detail:
|
Created Date: 2007-01-19 Completed Date: 2007-03-28 Revised Date: 2010-09-15 |
Medline Journal Info:
|
Nlm Unique ID: 101223209 Medline TA: AAPS J Country: United States |
Other Details:
|
Languages: eng Pagination: E655-60 Citation Subset: IM |
Affiliation:
|
Center for Drug Discovery, Northeastern University, Boston, MA, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Chromatography, Liquid
/
methods Endocannabinoids / chemistry, metabolism* Fatty Acids / analysis, chemistry, metabolism* Mass Spectrometry / methods |
| Chemical | |
Reg. No./Substance:
|
0/Endocannabinoids; 0/Fatty Acids |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: NAD metabolism and sirtuins: metabolic regulation of protein deacetylation in stress and toxicity.
Next Document: Conformational characteristics of the interaction of SR141716A with the CB1 cannabinoid receptor as ...