Document Detail


Endocannabinoid metabolism in human glioblastomas and meningiomas compared to human non-tumour brain tissue.
MedLine Citation:
PMID:  15816853     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The endogenous levels of the two cannabinoid receptor ligands 2-arachidonoyl glycerol and anandamide, and their respective congeners, monoacyl glycerols and N-acylethanolamines, as well as the phospholipid precursors of N-acylethanolamines, were measured by gas chromatography-mass spectrometry in glioblastoma (WHO grade IV) tissue and meningioma (WHO grade I) tissue and compared with human non-tumour brain tissue. Furthermore, the metabolic turnover of N-acylethanolamines was compared by measurements of the enzymatic activity of N-acyltransferase, N-acylphosphatidylethanolamine-hydrolysing phospholipase D and fatty acid amide hydrolase in the same three types of tissue. Glioblastomas were characterized by enhanced levels of N-acylethanolamines (eightfold, 128 +/- 59 pmol/micromol lipid phosphorus) including anandamide (17-fold, 4.6 +/- 3.1 pmol/micromol lipid phosphorus) and several species of N-acylphosphatidylethanolamines (three to eightfold). This was accompanied by a more than 60% reduction in the enzyme activities of N-acylphosphatidylethanolamine-hydrolysing phospholipase D and fatty acid amide hydrolase. By contrast, meningiomas were characterized by a massively enhanced level of 2-monoacyl glycerols (20-fold, 2293 +/- 361 pmol/micromol lipid phosphorus) including 2-arachidonoyl glycerol (20-fold, 1524 +/- 361 pmol/micromol lipid phosphorus). This was accompanied by an enhanced in vitro conversion of phosphatidylcholine to monoacyl glycerol (fivefold). The enhanced level of the 2-arachidonoyl glycerol, anandamide and other N-acylethanolamines detected in the two types of tumour tissue may possibly act as endogenous anti-tumour mediators by stimulation of both cannabinoid and non-cannabinoid receptor-mediated mechanisms.
Authors:
Gitte Petersen; Birthe Moesgaard; Patricia C Schmid; Harald H O Schmid; Helle Broholm; Michael Kosteljanetz; Harald S Hansen
Related Documents :
17600303 - The components required for amino acid neurotransmitter signaling are present in adipos...
10794723 - Net release of individual fatty acids from white adipose tissue during lipolysis in vit...
24441033 - An amino acid substitution-selection model adjusts residue fitness to improve phylogene...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neurochemistry     Volume:  93     ISSN:  0022-3042     ISO Abbreviation:  J. Neurochem.     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-04-08     Completed Date:  2005-05-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  England    
Other Details:
Languages:  eng     Pagination:  299-309     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, The Danish University of Pharmaceutical Sciences, Copenhagen, Denmark.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Brain / metabolism*,  pathology
Brain Neoplasms / metabolism*,  pathology
Endocannabinoids / metabolism*
Glioblastoma / metabolism*,  pathology
Humans
Meningioma / metabolism*,  pathology
Chemical
Reg. No./Substance:
0/Endocannabinoids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Activated mitogen-activated protein kinase kinase 7 redistributes to the cytosol and binds to Jun N-...
Next Document:  CGS21680 attenuates symptoms of Huntington's disease in a transgenic mouse model.