Document Detail


Endo-β-N-acetylglucosaminidases from infant gut-associated bifidobacteria release complex N-glycans from human milk glycoproteins.
MedLine Citation:
PMID:  22745059     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Breastfeeding is one of the main factors guiding the composition of the infant gut microbiota in the first months of life. This process is shaped in part by the high amounts of human milk oligosaccharides that serve as a carbon source for saccharolytic bacteria such as Bifidobacterium species. Infant-borne bifidobacteria have developed various molecular strategies for utilizing these oligosaccharides as a carbon source. We hypothesized that these species also interact with N-glycans found in host glycoproteins that are structurally similar to free oligosaccharides in human milk. Endo-β-N-acetylglucosaminidases were identified in certain isolates of Bifidobacterium longum subsp. longum, B. longum subsp. infantis, and Bifidobacterium breve, and their presence correlated with the ability of these strains to deglycosylate glycoproteins. An endoglycosidase from B. infantis ATCC 15697, EndoBI-1, was active toward all major types of N-linked glycans found in glycosylated proteins. Its activity was not affected by core fucosylation or extensive fucosylation, antenna number, or sialylation, releasing several N-glycans from human lactoferrin and immunoglobulins A and G. Extensive N-deglycosylation of whole breast milk was also observed after coincubation with this enzyme. Mutation of the active site of EndoBI-1 did not abolish binding to N-glycosylated proteins, and this mutant specifically recognized Man(3)GlcNAc(2)(α1-6Fuc), the core structure of human N-glycans. EndoBI-1 is constitutively expressed in B. infantis, and incubation of the bacterium with human or bovine lactoferrin led to the induction of genes associated to import and consumption of human milk oligosaccharides, suggesting linked regulatory mechanisms among these glycans. This work reveals an unprecedented interaction of bifidobacteria with host N-glycans and describes a novel endoglycosidase with broad specificity on diverse N-glycan types, potentially a useful tool for glycoproteomics studies.
Authors:
Daniel Garrido; Charles Nwosu; Santiago Ruiz-Moyano; Danielle Aldredge; J Bruce German; Carlito B Lebrilla; David A Mills
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-06-27
Journal Detail:
Title:  Molecular & cellular proteomics : MCP     Volume:  11     ISSN:  1535-9484     ISO Abbreviation:  Mol. Cell Proteomics     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-09-03     Completed Date:  2013-02-26     Revised Date:  2013-09-03    
Medline Journal Info:
Nlm Unique ID:  101125647     Medline TA:  Mol Cell Proteomics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  775-85     Citation Subset:  IM    
Affiliation:
Department of Viticulture & Enology, University of California Davis, Davis, California 95616, USA.
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MeSH Terms
Descriptor/Qualifier:
Bifidobacterium / enzymology*,  isolation & purification,  metabolism
Gastrointestinal Tract / microbiology*
Glycoproteins / chemistry,  metabolism*
Humans
Infant
Infant, Newborn
Lactoferrin / metabolism
Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase / metabolism*
Metagenome
Milk, Human / chemistry,  metabolism*
Polysaccharides / metabolism*
Grant Support
ID/Acronym/Agency:
R01AT007079/AT/NCCAM NIH HHS; R01HD061923/HD/NICHD NIH HHS; R01HD065122/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Glycoproteins; 0/Polysaccharides; EC 3.2.1.96/Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; EC 3.4.21.-/Lactoferrin
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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