Document Detail


Endcaps for stabilizing short DNA duplexes.
MedLine Citation:
PMID:  14565484     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The syntheses of endcaps for covalently linking the 3' and 5' hydroxyl groups of blunt end double-stranded DNA are described. Endcap diols were converted into DMTr protected phosphoramidites and incorporated between nucleotides 4 and 5 of a self-complementary octamer. The stabilizing effect of the endcaps on duplex DNA was determined by Tm experiments on the self-complementary octamer.
Authors:
Pei-Sze Ng; Maneesh R Pingle; Ganesan Balasundarum; Alan Friedman; Xiaolin Zu; Donald E Bergstrom
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Nucleosides, nucleotides & nucleic acids     Volume:  22     ISSN:  1525-7770     ISO Abbreviation:  Nucleosides Nucleotides Nucleic Acids     Publication Date:    2003 May-Aug
Date Detail:
Created Date:  2003-10-20     Completed Date:  2003-12-11     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  100892832     Medline TA:  Nucleosides Nucleotides Nucleic Acids     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1635-7     Citation Subset:  IM    
Affiliation:
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, Indiana 47907-1333, USA.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
DNA / chemistry*
Drug Stability
Models, Molecular
Nucleic Acid Conformation
Nucleic Acid Denaturation
Thermodynamics
Grant Support
ID/Acronym/Agency:
P30 CA23168/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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