Document Detail


Endangered wolves cloned from adult somatic cells.
MedLine Citation:
PMID:  17386020     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Over the world, canine species, including the gray wolf, have been gradually endangered or extinct. Many efforts have been made to recover and conserve these canids. The aim of this study was to produce the endangered gray wolf with somatic cell nuclear transfer (SCNT) for conservation. Adult ear fibroblasts from a female gray wolf (Canis lupus) were isolated and cultured in vitro as donor cells. Because of limitations in obtaining gray wolf matured oocytes, in vivo matured canine oocytes obtained by flushing the oviducts from the isthmus to the infundibulum were used. After removing the cumulus cells, the oocyte was enucleated, microinjected, fused with a donor cell, and activated. The reconstructed cloned wolf embryos were transferred into the oviducts of the naturally synchronized surrogate mothers. Two pregnancies were detected by ultrasonography at 23 days of gestation in recipient dogs. In each surrogate dog, two fetal sacs were confirmed by early pregnancy diagnosis at 23 days, but only two cloned wolves were delivered. The first cloned wolf was delivered by cesarean section on October 18, 2005, 60 days after embryo transfer. The second cloned wolf was delivered on October 26, 2005, at 61 days postembryo transfer. Microsatellite analysis was performed with genomic DNA from the donor wolf, the two cloned wolves, and the two surrogate female recipients to confirm the genetic identity of the cloned wolves. Analysis of 19 microsatellite loci confirmed that the cloned wolves were genetically identical to the donor wolf. In conclusion, we demonstrated live birth of two cloned gray wolves by nuclear transfer of wolf somatic cells into enucleated canine oocyte, indicating that SCNT is a practical approach for conserving endangered canids.
Authors:
Min Kyu Kim; Goo Jang; Hyun Ju Oh; Fibrianto Yuda; Hye Jin Kim; Woo Suk Hwang; Mohammad Shamim Hossein; Joung Joo Kim; Nam Shik Shin; Sung Keun Kang; Byeong Chun Lee
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cloning and stem cells     Volume:  9     ISSN:  1536-2302     ISO Abbreviation:  Cloning Stem Cells     Publication Date:  2007  
Date Detail:
Created Date:  2007-03-27     Completed Date:  2007-06-01     Revised Date:  2008-03-26    
Medline Journal Info:
Nlm Unique ID:  101125444     Medline TA:  Cloning Stem Cells     Country:  United States    
Other Details:
Languages:  eng     Pagination:  130-7     Citation Subset:  IM    
Affiliation:
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cloning, Organism*
Dogs
Extinction, Biological
Female
Fibroblasts* / cytology
Genome / genetics
Male
Microsatellite Repeats / genetics
Nuclear Transfer Techniques*
Oocytes*
Pregnancy
Wolves* / genetics
Comments/Corrections
Erratum In:
Cloning Stem Cells. 2007 Autumn;9(3):450

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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