| End-column chemiluminescence detection for pressurized capillary electrochromatographic analysis of norepinephrine and epinephrine. | |
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MedLine Citation:
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PMID: 17905257 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A simple and convenient end-column chemiluminescence (CL) detection coupled to pressurized capillary electrochromatography (pCEC) was described. Luminol and N-(4-aminobutyl)-N-ethylisoluminol (ABEI) were adopted as mode compounds to evaluate the feasibility of end-column reactor. Detailed analysis of ABEI revealed that the high sensitivity could be obtained with the reactor. Furthermore, determination of norepinephrine (NE) and epinephrine (EP), which were labeled with ABEI, was accomplished by using the end-column pCEC-CL detection based on ABEI-potassium ferricyanide-alkaline medium CL reaction system. Under the optimum conditions, the detection limit (S/N=3) of NE and EP was 0.08 microM and 0.06 microM, respectively. The proposed method has also been successfully applied to the analysis of adrenaline hydrochloride injection sample. |
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Authors:
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Zian Lin; Xiaoping Wu; Xucong Lin; Zenghong Xie |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't Date: 2007-09-14 |
Journal Detail:
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Title: Journal of chromatography. A Volume: 1170 ISSN: 0021-9673 ISO Abbreviation: J Chromatogr A Publication Date: 2007 Nov |
Date Detail:
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Created Date: 2007-10-15 Completed Date: 2008-02-26 Revised Date: 2009-01-15 |
Medline Journal Info:
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Nlm Unique ID: 9318488 Medline TA: J Chromatogr A Country: Netherlands |
Other Details:
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Languages: eng Pagination: 118-21 Citation Subset: IM |
Affiliation:
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Department of Chemistry, Fuzhou University, Fuzhou 350002, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Capillary Electrochromatography
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methods* Chemiluminescent Measurements / instrumentation*, methods* Epinephrine / analysis* Ferricyanides / chemistry Indicators and Reagents / chemistry Luminol / analogs & derivatives, chemistry Norepinephrine / analysis* Pressure Reproducibility of Results Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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0/Ferricyanides; 0/Indicators and Reagents; 13746-66-2/potassium ferricyanide; 51-41-2/Norepinephrine; 51-43-4/Epinephrine; 521-31-3/Luminol; 66612-29-1/6-(N-(4-aminobutyl)-N-ethyl)amino-2,3-dihydrophthalazine-1,4-dione |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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