Document Detail


Encystment and alkylresorcinol production by Azotobacter vinelandii strains impaired in poly-beta-hydroxybutyrate synthesis.
MedLine Citation:
PMID:  12732928     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The lipids poly-beta-hydroxybutyrate (PHB) and alkylresorcinols are the major metabolic products of Azotobacter vinelandii cysts. Cysts are formed in less than 0.01% of late stationary phase cells grown on sucrose. Culturing vegetative cells in n-butanol or beta-hydroxybutyrate induces encystment. After induction of encystment, PHB rapidly accumulates in large granules. Then, the cells begin the synthesis of alkylresorcinols that replace the phospholipids in the membranes and are components of the exine, the outer layer of the cyst envelope. Vegetative cells do not synthesize alkylresorcinols. We report here the effect of mutations in the phbBAC operon, coding for the enzymes of the PHB biosynthetic pathway, on the synthesis of alkylresorcinols and cyst formation. The phb mutations did not impair the capacity to form mature cysts. However, the cysts formed by these strains posses a thicker exine layer and a higher content of alkylresorcinols than the cysts formed by the wild-type strain. A blockage of PHB synthesis caused by phb mutations resulted in the synthesis of alkylresorcinols and encystment even under non-inducing conditions. We propose that, as a consequence of the blockage in the PHB biosynthetic pathway, the acetyl-CoA and reducing power pools are increased causing the shift to lipid metabolism required for the synthesis of alkylresorcinols and cyst formation.
Authors:
Daniel Segura; Tania Cruz; Guadalupe Espín
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2003-05-06
Journal Detail:
Title:  Archives of microbiology     Volume:  179     ISSN:  0302-8933     ISO Abbreviation:  Arch. Microbiol.     Publication Date:  2003 Jun 
Date Detail:
Created Date:  2003-05-29     Completed Date:  2003-08-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0410427     Medline TA:  Arch Microbiol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  437-43     Citation Subset:  IM    
Affiliation:
Instituto de Biotecnología, Universidad Nacional Autónoma de México, Departamento de Microbiología Molecular, Apartado Postal 510-3, 62271 Cuernavaca, Morelos, México.
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MeSH Terms
Descriptor/Qualifier:
Azotobacter vinelandii / cytology,  growth & development*,  metabolism*
Hydroxybutyrates / analysis,  metabolism*
Models, Genetic
Mutation
Phenotype
Polyesters / analysis,  metabolism*
Resorcinols / analysis,  isolation & purification,  metabolism*
Spores, Bacterial / metabolism,  ultrastructure
Chemical
Reg. No./Substance:
0/Hydroxybutyrates; 0/Polyesters; 0/Resorcinols; 26063-00-3/poly-beta-hydroxybutyrate

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