Document Detail

Encystment and alkylresorcinol production by Azotobacter vinelandii strains impaired in poly-beta-hydroxybutyrate synthesis.
MedLine Citation:
PMID:  12732928     Owner:  NLM     Status:  MEDLINE    
The lipids poly-beta-hydroxybutyrate (PHB) and alkylresorcinols are the major metabolic products of Azotobacter vinelandii cysts. Cysts are formed in less than 0.01% of late stationary phase cells grown on sucrose. Culturing vegetative cells in n-butanol or beta-hydroxybutyrate induces encystment. After induction of encystment, PHB rapidly accumulates in large granules. Then, the cells begin the synthesis of alkylresorcinols that replace the phospholipids in the membranes and are components of the exine, the outer layer of the cyst envelope. Vegetative cells do not synthesize alkylresorcinols. We report here the effect of mutations in the phbBAC operon, coding for the enzymes of the PHB biosynthetic pathway, on the synthesis of alkylresorcinols and cyst formation. The phb mutations did not impair the capacity to form mature cysts. However, the cysts formed by these strains posses a thicker exine layer and a higher content of alkylresorcinols than the cysts formed by the wild-type strain. A blockage of PHB synthesis caused by phb mutations resulted in the synthesis of alkylresorcinols and encystment even under non-inducing conditions. We propose that, as a consequence of the blockage in the PHB biosynthetic pathway, the acetyl-CoA and reducing power pools are increased causing the shift to lipid metabolism required for the synthesis of alkylresorcinols and cyst formation.
Daniel Segura; Tania Cruz; Guadalupe Espín
Related Documents :
1818188 - Role of the zymolyase-sensitive cyst wall of pneumocystis carinii in the oxidative burs...
17351948 - Ciliated hepatic foregut cyst: report of a case on fine-needle aspiration.
21699618 - Contrast harmonic ultrasound appearance of consecutive percutaneous renal biopsies in d...
10980478 - Guess what! eruptive vellus hair cysts.
655918 - Leber's congenital amaurosis. a retrospective study of 33 cases and a histopathological...
21680268 - Prevalence and clinical significance of pancreatic cysts associated with cysts in other...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2003-05-06
Journal Detail:
Title:  Archives of microbiology     Volume:  179     ISSN:  0302-8933     ISO Abbreviation:  Arch. Microbiol.     Publication Date:  2003 Jun 
Date Detail:
Created Date:  2003-05-29     Completed Date:  2003-08-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0410427     Medline TA:  Arch Microbiol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  437-43     Citation Subset:  IM    
Instituto de Biotecnología, Universidad Nacional Autónoma de México, Departamento de Microbiología Molecular, Apartado Postal 510-3, 62271 Cuernavaca, Morelos, México.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Azotobacter vinelandii / cytology,  growth & development*,  metabolism*
Hydroxybutyrates / analysis,  metabolism*
Models, Genetic
Polyesters / analysis,  metabolism*
Resorcinols / analysis,  isolation & purification,  metabolism*
Spores, Bacterial / metabolism,  ultrastructure
Reg. No./Substance:
0/Hydroxybutyrates; 0/Polyesters; 0/Resorcinols; 26063-00-3/poly-beta-hydroxybutyrate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Stimulation of GABAB receptors increases the expression of the proenkephalin gene in slice cultures ...
Next Document:  Serum ferritin and survival of renal transplant recipients: a prospective 10-year cohort study.