Document Detail

Encephalomyocarditis (EMC) virus infection in PC12 and C6 cells.
MedLine Citation:
PMID:  10319021     Owner:  NLM     Status:  MEDLINE    
PC12 cells derived rom rat pheochromocytoma and C6 cells derived from rat glioma were infected with 0.3 plaque forming units (PFU)/cell of the D variant of encephalomyocarditis virus (EMC-D), after pretreatment with or without nerve growth factor (NGF). The virus titres in medium and cells were investigated at 6, 12, 24, 48 and 72 h post infection (HPI), and histopathology and viral antigens in cells were examined at 24 and 48 HPI, respectively. As a result, neither viral replication nor light and electron microscopic changes were observed in PC12 cell cultures without NGF-pretreatment. On the contrary, in PC12 cell cultures with NGF-pretreatment, the virus titre prominently increased at 12 HPI, and peaked at 48 HPI. In addition, distinct histological and ultrastructural changes with viral antigens in cells were observed. C6 cells showed similar morphology and susceptibility to EMC-D-infection irrespective of NGF-pretreatment. Namely, the virus titres in C6 cell cultures increased slightly and viral antigens were found in a small number of C6 cells, but there were no evident histological and ultrastructural changes. These results suggest that PC12 cells pretreated with NGF and C6 cells are susceptible to EMC-D infection in vitro.
W Su; A Ueno-Yamanouchi; H Nakayama; K Doi
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of experimental pathology     Volume:  79     ISSN:  0959-9673     ISO Abbreviation:  Int J Exp Pathol     Publication Date:  1998 Dec 
Date Detail:
Created Date:  1999-05-17     Completed Date:  1999-05-17     Revised Date:  2011-12-21    
Medline Journal Info:
Nlm Unique ID:  9014042     Medline TA:  Int J Exp Pathol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  411-6     Citation Subset:  IM    
Department of Veterinary Pathology, Faculty of Agriculture, University of Tokyo, Japan.
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MeSH Terms
Antigens, Viral / analysis
Cardiovirus Infections / immunology,  pathology*,  virology
Encephalomyocarditis virus / immunology,  physiology*
Fluorescent Antibody Technique, Indirect
Glioma / ultrastructure
Nerve Growth Factors / pharmacology
Neurons / virology
PC12 Cells
Tumor Cells, Cultured / ultrastructure
Virus Replication
Reg. No./Substance:
0/Antigens, Viral; 0/Nerve Growth Factors

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