| Encephalitogenic activity of truncated myelin oligodendrocyte glycoprotein (MOG) peptides and their recognition by CD8+ MOG-specific T cells on oligomeric MHC class I molecules. | |
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MedLine Citation:
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PMID: 12578856 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have previously demonstrated that the 21-residue peptide pMOG(35-55) from myelin oligodendrocyte glycoprotein (MOG) contains an antigenic epitope that activates CD8(+) encephalitogenic T cells in C57BL/6 (B6) mice. To identify the core encephalitogenic epitope of CD8(+) MOG-specific T cells, we have prepared a panel of highly purified peptides of varying lengths, which span the entire length of pMOG(35-55), and tested their binding to recombinant H-2D(b) dimers and their ability to induce EAE. Two of the truncated peptides, pMOG(40-54) and pMOG(44-54), strongly bound recombinant H-2D(b) protein and this complex bound MOG-specific CD8(+) T cells. Interestingly, pMOG(40-54) retained the full capability of inducing paralytic disease, whereas only a part of the B6 mice immunized with pMOG(44-54) developed clinical paralysis and central nervous system (CNS) inflammation. Further deletion of 1 amino acid from either the N- or C-terminus of the peptide pMOG(44-54) dramatically reduced binding to recombinant H-2D(b), and abolished the induction of paralysis and CNS inflammation. Our results demonstrate that the ability of truncated pMOG(35-55) peptides to bind recombinant H-2D(b) protein does not always correlate with their ability of inducing encephalomyelitis. This approach enables the further identification of the core pathogenic epitope within the pMOG(35-55) that activates MOG-specific encephalitogenic CD8(+) T cells. |
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Authors:
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Deming Sun; Yiping Zhang; Bingyuan Wei; Stephen C Peiper; Hui Shao; Henry J Kaplan |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: International immunology Volume: 15 ISSN: 0953-8178 ISO Abbreviation: Int. Immunol. Publication Date: 2003 Feb |
Date Detail:
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Created Date: 2003-02-11 Completed Date: 2004-01-06 Revised Date: 2011-08-23 |
Medline Journal Info:
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Nlm Unique ID: 8916182 Medline TA: Int Immunol Country: England |
Other Details:
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Languages: eng Pagination: 261-8 Citation Subset: IM |
Affiliation:
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Kentucky Lions Eye Center, Department of Ophthalmology, and Vision Sciences, University of Louisville, Louisville, KY 40202, USA. d0sun001@louisville.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals CD8-Positive T-Lymphocytes / metabolism* Encephalitis / metabolism* Histocompatibility Antigens Class I / metabolism* Hybridomas / metabolism Mice Myelin-Associated Glycoprotein / metabolism* Peptides / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Histocompatibility Antigens Class I; 0/Myelin-Associated Glycoprotein; 0/Peptides; 0/myelin oligodendrocyte glycoprotein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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